LOCUS       sequence-93423-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA 
            intein, flanked by ITRs for AAV packaging. Combine with C-Terminal 
            Split Cas9 Gyra Intein for full length SpCas9 nickase production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G
  TITLE     Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human 
            cells using compact expression cassettes.
  JOURNAL   Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777.
  PUBMED    26126518
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-07-1"; volume: "5:10777. doi"; pages: " 
            10.1038/srep10777"
COMMENT     Sequence Label: N-Terminal Split Cas9 D10A Nickase with GyrA i...
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attcttagcg agcgaagcca gcgcaatcca cactaaatgc ggacctttgg atcactgcat
       61 agtctccggg ttttatctca tcaatcagtt tccacagcag ggtggggacg ccggcaacat
      121 ccaccagaca caaaaggggg tggttggctg tgcctgtcac tctcaggcct tctacggtgc
      181 ggacggtata cacaggatgt tcaccactgt ggaagagtct atcggccaga acaggattgc
      241 cgtggcggtc aagcaccttc aggtcgatgg cgttatcgct attagggcgg gcgcccggga
      301 cgatgtcagc gatgcgcact gattcgcctt cgggcagcgc caccagagca tcaccggtga
      361 tacagtatct ccgccgcttc agctgcttca tcactttgtc gtcgaacagg tgggcatagg
      421 ttttcagccg ttcctcgatc atctctctgt cctcaaacag tgtcagggtc agcacgatat
      481 cttccagaat gtcctcgttt tcctcattgt ccaggaagtc cttgtccttg ataattttca
      541 gcagatcgtg gtatgtgccc agggaggcgt tgaaccgatc ttccacgccg gagatttcca
      601 cggagtcgaa gcactcgatt ttcttgaagt agtcctcttt cagctgcttc acggtcactt
      661 tccggttggt cttgaacagc aggtccacga tggccttttt ctgctcgccg ctcaggaagg
      721 cgggctttct cattccctcg gtcacgtatt tcactttggt cagctcgtta tacacggtga
      781 agtactcgta cagcaggctg tgcttgggca gcaccttctc gttgggcagg ttcttatcga
      841 agttggtcat ccgctc
//
