LOCUS       sequence-93424-e         567 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA 
            intein, flanked by ITRs for AAV packaging. Combine with C-Terminal 
            Split Cas9 Gyra Intein for full length SpCas9 nickase production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G
  TITLE     Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human 
            cells using compact expression cassettes.
  JOURNAL   Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777.
  PUBMED    26126518
REFERENCE   2  (bases 1 to 567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-07-1"; volume: "5:10777. doi"; pages: " 
            10.1038/srep10777"
COMMENT     Sequence Label: N-Terminal Split Cas9 D10A Nickase with GyrA i...
FEATURES             Location/Qualifiers
     source          1..567
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(315..335)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(339..365)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcttccagtc tgtggaagaa gctgtcgtcc accttggcca tctcgttgct gaagatctct
       61 tgcagatagc agatccggtt cttccgtctg gtgtatcttc ttctggcggt tctcttcagc
      121 cgggtggcct cggctgtttc gccgctgtcg aacagcaggg ctccgatcag gttcttcttg
      181 atgctgtgcc ggtcggtgtt gcccagcacc ttgaatttct tgctgggcac cttgtactcg
      241 tcggtgatca cggcccagcc cacagagttg gtgccgatgg ccaggccgat gctgtacttc
      301 ttgtcggacg cttcgacctt gcgctttttc ttcggcgaag cgtaatctgg aacatcgtat
      361 gggtacatgg tggcaccggt ccaacctgaa aaaaagtgat ttcaggcagg tgctccaggt
      421 aattaaacat taatacccca ccaaccaacc atcccttaaa cccttacctc ttgctcagct
      481 aattacagcc cggaggagaa gggccgtccc gcccgctcac ctgtgggagt aacgcggtca
      541 gtcagagccg gggcgggcgg cgcgagg
//
