LOCUS       sequence-93427-e         501 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon optimized Cas9 nuclease from Streptococcus pyogenes 
            (Cbh-3X-FLAG-NLS-SpCas9-NLS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. 
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.143"; journalName: "Nat Protoc"; date: 
            "2013-11"; volume: "8"; issue: "11"; pages: "2281-308"
COMMENT     Sequence Label: pSpCas9 (PX165)
FEATURES             Location/Qualifiers
     source          1..501
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct
       61 gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt
      121 gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa
      181 ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct
      241 gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac
      301 ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga
      361 cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct
      421 gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa
      481 caccgagatc accaaggccc c
//
