LOCUS       sequence-9343-ex        2000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Nausch LW, Ledoux J, Bonev AD, Nelson MT, Dostmann WR
  TITLE     Differential patterning of cGMP in vascular smooth muscle cells 
            revealed by single GFP-linked biosensors.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):365-70.
  PUBMED    18165313
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Jan 8. 105(1):365-70."
COMMENT     Sequence Label: pSP72-deltaFlincG
FEATURES             Location/Qualifiers
     source          1..2000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1095..1116
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1222..1243)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1483..1502)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(1701..1720)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1702..1720)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1807..1824)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acgtaacatg catgccntgc aggtcgactc tagaggatcc ggaccatgct ccgacaggca
       61 ttccggaagt tcaccaaatc cgaaaggtcc aaggatctca taaaggaggc catccttgac
      121 aatgacttta tgaagaactt ggagctgtca cagatccaag agattgtgga ttgtatgtac
      181 ccagtggagt acggcaaaga cagctgcatc atcaaagaag gagatgtggg gtcactggtg
      241 tatgtcatgg aagatggtaa ggttgaagtt acaaaagaag gcgtgaagct gtgcacaatg
      301 ggtcctggta aagtgtttgg agagttggct atcctttaca actgtacccg gacggcgacc
      361 gtcaaaactc ttgtaaatgt gaaactctgg gccattgatc gacaatgttt tcagacgata
      421 atgatgagga caggacttat caagcatacc gagtatatgg aatttttaaa aagcgttcca
      481 acattccaga gccttcctga agagatcctc agtaagcttg ctgacgtcct tgaagagacc
      541 cactatgaaa atggggaata tatcatcagg caaggtgcaa gaggggacac cttctttatc
      601 atcagtaaag gaaaggttaa tgtcactcgt gaagactcgc ccaatgaaga cccagtcttt
      661 cttagaacct taggaaaagg agattggttt ggagagaaag ccttgcaggg ggaagatgtg
      721 agaacagcga atgtaattgc ggcagaagct gtaacctgcc ttgtgatcga cagagactct
      781 ttcaaacatt tgattggagg attagatgat gtttctaata aagcatatga agatgcagaa
      841 gctaaggcaa aatatgaagc tgaagctgct ttcttcgcca acctgaagaa cgtctatatc
      901 atggccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag
      961 gacggcggcg tgcagctcgc ctaccactac cagcagaaca cccccatcgg cgacggcccc
     1021 gtgctgctgc ccgacaacca ctacctgagc acccagtcca aactttcgaa agaccccaac
     1081 gagaagtgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1141 atggacgagc tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg cgaggagctg
     1201 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1261 agcgtgtccg gcgagggtga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1321 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1381 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1441 atgcccgaag gctacatcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1501 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1561 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cctgtctgat
     1621 ttaacatcat tgacaccctt ggagttggag gtttcggacg agtagaacga attcatcgat
     1681 gatatcagat ctgccggtct ccctatagtg agtcgtatta atttcgataa gccaggttaa
     1741 cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     1801 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1861 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1921 gtgagcaaaa ggccagcaaa aagccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1981 ccataagctc cgcccccctg
//
