LOCUS       sequence-9344-ex        2794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Nausch LW, Ledoux J, Bonev AD, Nelson MT, Dostmann WR
  TITLE     Differential patterning of cGMP in vascular smooth muscle cells 
            revealed by single GFP-linked biosensors.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):365-70.
  PUBMED    18165313
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Jan 8. 105(1):365-70."
COMMENT     Sequence Label: pcDNA3.1(+)-deltaFlincG
FEATURES             Location/Qualifiers
     source          1..2794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1080..1101
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1207..1228)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1468..1487)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(2614..2631)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2620..2731
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcttggtac cgagctcgga tccggaccat ggcactccga caggcattcc ggaagttcac
       61 caaatccgaa aggtccaagg atctcataaa ggaggccatc cttgacaatg actttatgaa
      121 gaacttggag ctgtcacaga tccaagagat tgtggattgt atgtacccag tggagtacgg
      181 caaagacagc tgcatcatca aagaaggaga tgtggggtca ctggtgtatg tcatggaaga
      241 tggtaaggtt gaagttacaa aagaaggcgt gaagctgtgc acaatgggtc ctggtaaagt
      301 gtttggagag ttggctatcc tttacaactg tacccggacg gcgaccgtca aaactcttgt
      361 aaatgtgaaa ctctgggcca ttgatcgaca atgttttcag acgataatga tgaggacagg
      421 acttatcaag cataccgagt atatggaatt tttaaaaagc gttccaacat tccagagcct
      481 tcctgaagag atcctcagta agcttgctga cgtccttgaa gagacccact atgaaaatgg
      541 ggaatatatc atcaggcaag gtgcaagagg ggacaccttc tttatcatca gtaaaggaaa
      601 ggttaatgtc actcgtgaag actcgcccaa tgaagaccca gtctttctta gaaccttagg
      661 aaaaggagat tggttgcgag agaagccttg cagggggaag atgtgagaac agcgaatata
      721 attgcggcag aagctgtaac ctgccttgtg atcgacagag actctttcaa acatttgatt
      781 ggaggattag atgatgtttc taataaagca tatgaagatg cagaagctaa ggcaaaatat
      841 gaagctgaag ctgctttctt cgccaacctg aagaacgtct atatcatggc cgacaagcag
      901 aagaacggca tcaaggcgaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
      961 ctcgcctacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1021 aaccactacc tgagcaccca gtccaaactt tcgaaagacc ccaacgagaa gtgcgatcac
     1081 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1141 aagggcggta ccggagggag catggtgagc aagggcgagg agctgttcac cggggtggtg
     1201 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1261 ggtgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1321 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     1381 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1441 atccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1501 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1561 gacggcaaca tcctggggca caagctggag tacaacctgt ctgatttaac atcattgaca
     1621 cccttggagt tggaggtttc ggacgagtag aactggtcca gttaaaaagt gaagaatcca
     1681 aaacctttgc aatgaagatt ctcaagaaac gccacatcgt ggatacaaga cagcaggaac
     1741 acatccgctc ggagaagcag atcatgcagg gggcccattc ggacttcata gtgagattat
     1801 acagaacatt taaggacagc aaatatttgt atatgttgat ggaagcttgc ctaggtggag
     1861 agctctggac cattctcagg gatcgggggt catttgaaga ttctacaacc agattttata
     1921 cagcatgtgt ggtagaagct tttgcctatc tgcattccaa aggaatcatt tacagggacc
     1981 tcaagcctga aaatctcatc ctagatcacc gaggttatgc caaactggtt gattttggct
     2041 ttgcaaagaa aataggattt ggaaagaaaa catggacttt ttgtgggact ccagaatatg
     2101 tagccccaga gatcatcctg aacaaaggcc atgacatttc agccgactat tggtcactgg
     2161 gaatcctcat gtatgagctt ctgactggca gcccaccttt ctcaggccca gatcctatga
     2221 aaacctataa catcatattg agggggattg acatgataga gtttccaaag aagattgcca
     2281 aaaatgctgc taatttaatt aaaaaactat gcagggataa tccatcaaaa agattaggga
     2341 atttgaaaaa cggagtgaaa gacattcaaa agcacaaatg gtttgagggc tttaattggg
     2401 aaggcttaag aaaaggcacc ttgacacctc ctataatacc aagtgttgca tcacccacag
     2461 acacaagcaa ttttgacagt ttccctgagg acaatgacga accgccacct gatgacaact
     2521 caggatggga catagacttc taagaattct gcagatatcc agcacagtgg cggccgctcg
     2581 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     2641 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     2701 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaagtgt cattctattc
     2761 tgggggggtg ggggggggca ggacaaccag gggg
//
