LOCUS       sequence-93449-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 551, Emission:
            565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, 
            Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, 
            Masai H, Miyawaki A
  TITLE     Visualizing spatiotemporal dynamics of multicellular cell-cycle 
            progression.
  JOURNAL   Cell. 2008 Feb 8;132(3):487-98. doi: 10.1016/j.cell.2007.12.033.
  PUBMED    18267078
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2007.12"; journalName: "Cell"; date: "2008-02-8- 8";
            volume: "132"; issue: "3"; pages: "487-98"
COMMENT     Sequence Label: mKO2-pBAD
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..669)
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             complement(676..696)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 agcttcgaat tcttagctat gagctactgc atcttctacc tgctcagtaa tgttgccttc
       61 ggttttcctg acgaggcgat ggccgatgta atggtctcct ggcatttcaa gaatctcttt
      121 tgccgccttg taagtagtct tcatttggca tttgtgattg ccgcctcctt caagttttag
      181 gtacatcgta acatcaccct tcagaactcc gtcgctggca gtaattttct cggttgatgg
      241 ctcccaatca acactttggt tttgcatgat aggaccatcg gcaggaaagt taaccccagt
      301 aaatttggat ttgtggtaga aggtgtttcc tctaaggctt atatgcgcac tgactgaagc
      361 ggacccacca tcttcgaact ccaacgacct ttcccatgac aggccttcag gaaatgcttg
      421 tttgaaatag tctggtatct cttctggata tttagtaaat actctgtggc cgtaacagaa
      481 cacgtgtgac actaagtcaa acgcgaaagg cattggcccg ccctcggcca ttgtgacgcg
      541 tagtgtcatc tcttgatgtc cctcgtaagg tctgcctgtg ccttcacctt caattgtgaa
      601 ctcatgccca ttgacggagc cgtccatgta gtacctcatc ttcatctctg gtttaatcac
      661 actcaccatg ctcgagccct ggaagtacag gttctcgtac agatcccgac c
//
