LOCUS       sequence-9345-ex        2349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Nausch LW, Ledoux J, Bonev AD, Nelson MT, Dostmann WR
  TITLE     Differential patterning of cGMP in vascular smooth muscle cells 
            revealed by single GFP-linked biosensors.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):365-70.
  PUBMED    18165313
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Jan 8. 105(1):365-70."
COMMENT     Sequence Label: pRSETA-alphaFlincG
FEATURES             Location/Qualifiers
     source          1..2349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             42..74
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(78..86,87..101)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     1403..1424
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1530..1551)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1791..1810)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atatacatat gcggggttct catcatcatc atcatcatgg tatggctagc atgactggtg
       61 gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatcgatgg ggatccgagc
      121 tcgaggcgat gagcgagctg gaggaagact ttgccaagat tctcatgctc aaggaggaga
      181 ggatcaaaga gctggagaag cggctgtcag agaaggagga agaaatccag gagctgaaga
      241 ggaaactcca taaatgccag tcagtgctgc ccgtgccctc gacccacatc ggcccccgga
      301 ccacccgggc acagggcatc tcggccgagc cgcagaccta caggtccttc cacgacctcc
      361 gacaggcatt ccggaagttc accaaatccg aaaggtccaa ggatctcata aaggaggcca
      421 tccttgacaa tgactttatg aagaacttgg agctgtcaca gatccaagag attgtggatt
      481 gtatgtaccc agtggagtac ggcaaagaca gctgcatcat caaagaagga gatgtggggt
      541 cactggtgta tgtcatggaa gatggtaagg ttgaagttac aaaagaaggc gtgaagctgt
      601 gcacaatggg tcctggtaaa gtgtttggag agttggctat cctttacaac tgtacccgga
      661 cggcgaccgt caaaactctt gtaaatgtga aactctgggc cattgatcga caatgttttc
      721 agacgataat gatgaggaca ggacttatca agcataccga gtatatggaa tttttaaaaa
      781 gcgttccaac attccagagc cttcctgaag agatcctcag taagcttgct gacgtccttg
      841 aagagaccca ctatgaaaat ggggaatata tcatcaggca aggtgcaaga ggggacacct
      901 tctttatcat cagtaaagga aaggttaatg tcactcgtga agactcgccc aatgaagacc
      961 cagtctttct tagaacctta ggaaaaggag attggtttgg agagaaagcc ttgcaggggg
     1021 aagatgtgag aacagcgaat gtaattgcgg cagaagctgt aacctgcctt gtgatcgaca
     1081 gagactcttt caaacatttg attggaggat tagatgatgt ttctaataaa gcatatgaag
     1141 atgcagaagc taaggcaaaa tatgaagctg aagctgcttt cttcgccaac ctgaagaacg
     1201 tctatatcat ggccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca
     1261 acatcgagga cggcggcgtg cagctcgcct accactacca gcagaacacc cccatcggcg
     1321 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaaa ctttcgaaag
     1381 accccaacga gaagtgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1441 ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg agcaagggcg
     1501 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1561 acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag ctgaccctga
     1621 agttcatctg caccaccggc aagctgcccg tgccctggcc cnccctcgtg accaccctga
     1681 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1741 agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag gacgacggca
     1801 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1861 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacc
     1921 tgtctgattt aacatcattg acactgtctg atttaacatc attgacaccc ttggagttgg
     1981 aggtttcgga cgagtagaac tggtccagtt aaaaagtgaa gaatccaaaa cctttgcaat
     2041 gaagattctc aagaaacgcc acatcgtgga tacaagacag caggaacaca tccgctcgga
     2101 gaagcagatc atgcaggggg cccattcgga cttcatagtg agattataca gaacatttaa
     2161 ggacagcaaa tatttgtata tgttgatgga agcttgccta ggtggagagc tctggaccat
     2221 tctcagggat cgggggtcat ttgaagattc tacaaccaga ttttatacag catgtgtggt
     2281 agaagctttt gcctatctgc nttccaaagg aatcatttac agggacctca agcctgaaaa
     2341 tctcatcct
//
