LOCUS       sequence-93451-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 472, Emission:
            495.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A
  TITLE     Cyan-emitting and orange-emitting fluorescent proteins as a 
            donor/acceptor pair for fluorescence resonance energy transfer.
  JOURNAL   Biochem J. 2004 Jul 1. 381(Pt 1):307-12.
  PUBMED    15065984
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2004 Jul 1. 381(Pt 1):307-12."
COMMENT     Sequence Label: MiCy-pBAD
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..711)
                     /codon_start=1
                     /product="dimeric Midoriishi-Cyan fluorescent protein"
                     /label=MiCy
                     /translation="MVSYSKQGIAQEMRTKYRMEGSVNGHEFTIEGVGTGNPYEGKQMS
                     ELVIIKPKGKPLPFSFDILSTAFQYGNRCFTKYPADMPDYFKQAFPDGMSYERSFLFED
                     GGVATASWSIRLEGNCFIHNSIYHGVNFPADGPVMKKQTIGWDKSFEKMSVAKEVLRGD
                     VTQFLLLEGGGYQRCRFHSTYKTEKPVAMPPSHVVEHQIVRTDLGQTAKGFKVKLEEHA
                     EAHVNPLKVK"
     CDS             complement(718..738)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(751..783)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(787..804)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcttcgaat tcttatttaa ccttcaaagg gttaacatga gcctcagcat gttcttccag
       61 cttgaccttg aagccttttg cagtttggcc aaggtcggtc ctcacaattt gatgttctac
      121 gacatgactc gggggcattg cgactggctt ctccgttttg taagtggagt gaaaccggca
      181 tctctggtaa ccacctcctt cgagcagaag aaactgagtc acatcacctc ttagcacctc
      241 tttagccaca ctcatttttt cgaaggactt atcccagcca attgtctgct tcttcattac
      301 gggtccatca gcgggaaagt ttacgccatg atagatggaa ttgtggatga agcaatttcc
      361 ttcgagacga atgctccagc tggctgtagc aactcctcca tcctcaaata gaaatgacct
      421 ttcatatgac attccatctg ggaatgcttg cttgaaatag tcaggcatgt cggcagggta
      481 ctttgtgaag catctgtttc catattgaaa ggctgttgac agtatgtcaa aggagaatgg
      541 aaggggtttt cccttaggct tgatgatcac taattcggac atctgtttcc cttcgtaagg
      601 gtttccagtt cctacacctt cgatcgtgaa ctcatggcca ttgacactgc cttccatacg
      661 gtatttcgtc cgcatttctt gtgcgatgcc ttgctttgaa taagacacca tgctcgagcc
      721 ctggaagtac aggttctcgt acagatcccg acccatttgc tgtccaccag tcatgctagc
      781 cataccatga tgatgatgat gatga
//
