LOCUS       sequence-93452-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 399, Emission:
            456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-pBAD
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             33..55
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             75..92
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             96..128
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             141..161
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctccataccc gtttttttgg gctagaaata attttgttta actttaagaa ggagatatac
       61 atatgcgggg ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc
      121 aaatgggtcg ggatctgtac gagaacctgt acttccaggg ctcgagcatg gtgtctaagg
      181 gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
      241 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
      301 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
      361 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
      421 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
      481 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
      541 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
      601 aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
      661 ccctgaagct cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca
      721 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
      781 ga
//
