LOCUS       sequence-93459-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 580, Emission:
            608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-pBAD
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             52..74
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             94..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..147
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             160..180
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa
       61 ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca tggtatggct
      121 agcatgactg gtggacagca aatgggtcgg gatctgtacg agaacctgta cttccagggc
      181 tcgagcatgg tgagcgagct gattaaggag aacatgccca tgaagctgta catggagggc
      241 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
      301 acccagacca tgagaatcaa ggtcgtcgag ggcggccctc tccccttcgc cttcgacatc
      361 ctggctacca gcttcatgta cggcagcaga accttcatca agcaccctcc gggcatcccc
      421 gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
      481 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
      541 aacgtcaagg ttagaggggt gaacttccca gccaacggcc ctgtgatgca gaagaaaaca
      601 ctcggctggg aggcctccac cgagacgatg taccccgctg acggcggcct ggaaggcgca
      661 tgtgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaacct tgagaccaca
      721 tacagatcca agaaacccgc tacgaacctc aagatgcccg gcgtctacaa cgtggaccac
      781 agactggaaa gaatc
//
