LOCUS       sequence-9346-ex        2409 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Nausch LW, Ledoux J, Bonev AD, Nelson MT, Dostmann WR
  TITLE     Differential patterning of cGMP in vascular smooth muscle cells 
            revealed by single GFP-linked biosensors.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):365-70.
  PUBMED    18165313
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Jan 8. 105(1):365-70."
COMMENT     Sequence Label: pRSETA-betaFlincG
FEATURES             Location/Qualifiers
     source          1..2409
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             54..86
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(90..98,99..113)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     1463..1484
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1590..1611)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1851..1870)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttaagaagg agatatacat atgcggggtt ctcatcatca tcatcatcat ggtatggcta
       61 gcatgactgg tggacagcaa atgggtcggg atctgtacga cgatgacgat aaggatcgat
      121 ggggatccga gctcgaggcg atgggcaccc tgcgggattt acagtacgcg ctccaggaga
      181 agatcgagga gctgaggcag cgggatgctc tcatcgacga gctggagctg gagttggatc
      241 agaaggacga actgatccag aagctgcaga acgagctgga caagtaccgc tcggtgatcc
      301 ggccggccac ccagcaggcg cagaagcaga gcgcgagcac cttgcaaggc gagccgcgca
      361 ccaagcggca ggcgatctcc gctgagccca ccgcctttga catccaggat ctcagccatg
      421 tgaccctgcc cttctacccc aagagtccac agtccaagga tctcataaag gaggccatcc
      481 ttgacaatga ctttatgaag aacttggagc tgtcacagat ccaagagatt gtggattgta
      541 tgtacccagt ggagtacggc aaagacagct gcatcatcaa agaaggagat gtggggtcac
      601 tggtgtatgt catggaagat ggtaaggttg aagttacaaa agaaggcgtg aagctgtgca
      661 caatgggtcc tggtaaagtg tttggagagt tggctatcct ttacaactgt acccggacgg
      721 cgaccgtcaa aactcttgta aatgtgaaac tctgggccat tgatcgacaa tgttttcaga
      781 cgataatgat gaggacagga cttatcaagc ataccgagta tatggaattt ttaaaaagcg
      841 ttccaacatt ccagagcctt cctgaagaga tcctcagtaa gcttgctgac gtccttgaag
      901 agacccacta tgaaaatggg gaatatatca tcaggcaagg tgcaagaggg gacaccttct
      961 ttatcatcag taaaggaaag gttaatgtca ctcgtgaaga ctcgcccaat gaagacccag
     1021 tctttcttag aaccttagga aaaggagatt ggtttggaga gaaagccttg cagggggaag
     1081 atgtgagaac agcgaatgta attgcggcag aagctgtaac ctgccttgtg atcgacagag
     1141 actctttcaa acatttgatt ggaggattag atgatgtttc taataaagca tatgaagatg
     1201 cagaagctaa ggcaaaatat gaagctgaag ctgctttctt cgccaacctg aagctgaacg
     1261 tctatatcat ggccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca
     1321 acatcgagga cggcggcgtg cagctcgcct accactacca gcagaacacc cccatcggcg
     1381 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaaa ctttcgaaag
     1441 accccaacga gaagtgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1501 ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg agcaagggcg
     1561 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1621 acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag ctgaccctga
     1681 agttcatctg caccaccggc aagctgcccg tgccctggcc cnccctcgtg accaccctga
     1741 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1801 agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag gacgacggca
     1861 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1921 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacc
     1981 tgtctgattt aacatcattg acactgtctg atttaacatc attgacaccc ttggagttgg
     2041 aggtttcgga cgagtagaac tggtccagtt aaaaagtgaa gaatccaaaa cctttgcaat
     2101 gaagattctc aagaaacgcc acatcgtgga tacaagacag caggaacaca tccgctcgga
     2161 gaagcagatc atgcaggggg cccattcgga cttcatagtg agattataca gaacatttaa
     2221 ggacagcaaa tatttgtata tgttgatgga agcttgccta ggtggagagc tctggaccat
     2281 tctcagggat cgggggtcat ttgaagattc tacaaccaga ttttatacag catgtgtggt
     2341 agaagctttt gcctatctgc nttccaaagg aatcatttac agggacctca agcctgaaaa
     2401 tctcatcct
//
