LOCUS       sequence-93462-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 516, Emission:
            529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY
  TITLE     Reducing the environmental sensitivity of yellow fluorescent 
            protein. Mechanism and applications.
  JOURNAL   J Biol Chem. 2001 Aug 3;276(31):29188-94. Epub 2001 May 31.
  PUBMED    11387331
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-08-3"; volume: "276(31)"; pages: "29188-94. Epub
            2001 May 31"
COMMENT     Sequence Label: mCitrine-pBAD
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..116
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(120..128,129..143)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(159..161,162..164,165..677)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(204..225)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(465..484)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctctactgtt tctccatacc cgttttttgg gctaacagga ggaattaacc atggggggtt
       61 ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa atgggtcggg
      121 atctgtacga cgatgacgat aaggatccga gctcgagcat ggtgagcaag ggcgaggagc
      181 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      241 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      301 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg
      361 gcctgatgtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      421 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      481 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      541 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
      601 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
      661 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
      721 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccaagc
      781 tgagcaaaga cc
//
