LOCUS       sequence-93466-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 559, Emission:
            600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-pBAD
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             53..75
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             95..112
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             116..148
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             161..181
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             188..898
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 atcgcaactc tctactgttt ctccataccc gtttttttgg gctagaaata attttgttta
       61 actttaagaa ggagatatac atatgcgggg ttctcatcat catcatcatc atggtatggc
      121 tagcatgact ggtggacagc aaatgggtcg ggatctgtac gagaacctgt acttccaggg
      181 ctcgagcatg gtgtctaagg gcgaagagct gatcaaggaa aatatgcgta tgaaggtggt
      241 catggaaggt tcggtcaacg gccaccaatt caaatgcaca ggtgaaggag aaggcaatcc
      301 gtacatggga actcaaacca tgaggatcaa agtcatcgag ggaggacccc tgccatttgc
      361 ctttgacatt cttgccacgt cgttcatgta tggcagccgt acttttatca agtacccgaa
      421 aggcattcct gatttcttta aacagtcctt tcctgagggt tttacttggg aaagagttac
      481 gagatacgaa gatggtggag tcgtcaccgt catgcaggac accagccttg aggatggctg
      541 tctcgtttac cacgtccaag tcagaggggt aaactttccc tccaatggtc ccgtgatgca
      601 gaagaagacc aagggttggg agcctaatac agagatgatg tatccagcag atggtggtct
      661 gaggggatac actcatatgg cactgaaagt tgatggtggt ggccatctgt cttgctcttt
      721 cgtaacaact tacaggtcaa aaaagaccgt cgggaacatc aagatgcccg gtatccatgc
      781 cgttgatcac cgcctggaaa ggttagagga aagtgacaat gaaatgttcg tagtacaacg
      841 cgaacacgca gttgccaagt tcgccgggct tggtggtggg atggacgagc tgtacaagta
      901 ag
//
