LOCUS       sequence-93475-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 604, Emission:
            659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-pBAD
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             52..74
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             94..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..147
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             160..180
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa
       61 ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca tggtatggct
      121 agcatgactg gtggacagca aatgggtcgg gatctgtacg agaacctgta cttccagggc
      181 tcgagcatgg tgagcaaggg cgaggagctg atcaaggaga acatgcacat gaagctgtac
      241 atggaaggca ccgtgaacaa ccaccacttc aagtgcacca ccgaagggga gggcaagccc
      301 tacgagggca cccagaccca gaggattaag gtggtggagg gaggccccct gccgttcgca
      361 ttcgacatcc tggccacctg ctttatgtac gggagcaaga ccttcatcaa ccacacccag
      421 ggcatccccg atttctttaa gcagtccttc cctgagggct tcacatggga gagagtcacc
      481 acatacgaag acgggggcgt gcttaccgtt acccaggaca ccagcctcca ggacggctgc
      541 ttgatctaca acgtcaagct cagaggggtg aacttcccat ccaacggccc tgtgatgcag
      601 aagaaaacac tcggctggga ggccaccacc gagaccctgt accccgctga cggcggcctg
      661 gaaggcagat gcgacatggc cctgaagctc gtgggcgggg gccacctgca ctgcaacctg
      721 aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctacttt
      781 gtggaccgca gactggaaag aatcaaggag gccgacaatg agacctacgt cgagcagcac
      841 gaggtggctg tggccagata ctgcgacctc c
//
