LOCUS       sequence-93479-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mitochondrial Zn-binding FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Park JG, Qin Y, Galati DF, Palmer AE
  TITLE     New Sensors for Quantitative Measurement of Mitochondrial Zn(2+).
  JOURNAL   ACS Chem Biol. 2012 Aug 10.
  PUBMED    22850482
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2012 Aug 10."
COMMENT     Sequence Label: pcDNA-mito-ZapCY1
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..135
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             157..231
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             253..327
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             349..423
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     complement(499..520)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cccactgcct tactggctta tcgaaattaa tacgcactca ctatagggag acccaagctt
       61 atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca
      121 gtgccgcgcg ccaagatcca ttcgttggga gatcctatgt ccgtcctgac gccgctgctg
      181 ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc cgcgcgccaa gatccattcg
      241 ttgggagatc ctatgtccgt cctgacgccg ctgctgctgc ggggcttgac aggctcggcc
      301 cggcggctcc cagtgccgcg cgccaagatc cattcgttgg gagatcctat gtccgtcctg
      361 acgccgctgc tgctgcgggg cttgacaggc tcggcccggc ggctcccagt gccgcgcgcc
      421 aagatccatt cgttggggaa gcttggggat cccatggtga gcaagggcga ggagctgttc
      481 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caggttcagc
      541 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      601 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg
      661 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      721 cccgaaggct acgtccagga gcgtaccatc ttcttc
//
