LOCUS       sequence-9348-ex       18591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18591)
  TITLE     Jacks Lab Unpublished Plasmids
REFERENCE   2  (bases 1 to 18591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRosa26 LSL luciferase targeting
FEATURES             Location/Qualifiers
     source          1..18591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..1089
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1165..1468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     gap             1597..1606
                     /estimated_length=10
     rep_origin      complement(1609..2064)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     2206..2222
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2232..2250
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2290..2323)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2864..3463)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2947..2967)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3444..3463
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3507..4006)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     intron          4616..4681
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4811..4831
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5256..5377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          5470..5535
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5665..5685
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6110..6231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          6324..6389
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6519..6539
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6964..7085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          7178..7243
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7373..7393
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7818..7939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      7964..7973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8071..8080
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8077..9726
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(8143..8161)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    9831..14100
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        14149..14642
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             14669..15238
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphteria toxin Fragment A"
                     /label=DTA
                     /translation="DDVVILLIFCMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDD
                     DWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELG
                     LSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEIN
                     FETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          15315..15380
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(15448..15465)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    15454..15678
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(15746..15766)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(15746..15764)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(15785..15801)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15785..15801)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15798..15820)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15809..15825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15833..15839,15840..15857,15858..15863))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15878..15899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(16016..16033)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16187..16775)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16267..16286)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16946..17737,17738..17806))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17569..17588
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17807..17911)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(17937..18392)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     18534..18550
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        18560..18578
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccccgcggca ggccctccga gcgtggtgga gccgttctgt gagacagccg ggtacgagtc
       61 gtgacgctgg aaggggcaag cgggtggtgg gcaggaatgc ggtccgccct gcagcaaccg
      121 gagggggagg gagaagggag cggaaaagtc tccaccggac gcggccatgg ctcggggggg
      181 ggggggcagc ggaggancgc ttccggccga cgtctcgtcg ctgattggct tnttttcctc
      241 ccgccgtgtg tgaaaacaca aatggcgtgt tttggttggc gtaaggcgcc tgtcagttaa
      301 cggcagccgg agtgcgcagc cgccggcagc ctcgctctgc ccactgggtg gggcgggagg
      361 taggtggggt gaggcgagct gnacgtgcgg gcgcggtcgg cctctggcgg ggcgggggag
      421 gggagggagg gtcagcgaaa gtagctcgcg cgcgagcggc cgcccaccct ccccttcctc
      481 tgggggagtc gttttacccg ccgccggccg ggcctcgtcg tctgattggc tctcggggcc
      541 cagaaaactg gcccttgcca ttggctcgtg ttcgtgcaag ttgagtccat ccgccggcca
      601 gcgggggcgg cgaggaggcg ctcccaggtt ccggccctcc cctcggcccc gcgccgcaga
      661 gtctggccgc gcgcccctgc gcaacgtggc aggaagcgcg cgctgggggc ggggacgggc
      721 agtagggctg agcggctgcg gggcgggtgc aagcacgttt ccgacttgag ttgcctcaag
      781 aggggcgtgc tgagccagac ctccatcgcg cactccgggg agtggaggga aggagcgagg
      841 gctcagttgg gctgttttgg aggcaggaag cacttgctct cccaaagtcg ctctgagttg
      901 ttatcagtaa gggagctgca gtggagtagg cggggagaag gccgcaccct tctccggagg
      961 ggggagggga gtgttgcaat acctttctgg gagttctctg ctgcctcctg gcttctgagg
     1021 accgccctgg gcctgggaga atcccttgcc ccctcttccc ctcgtgatct gcaactccag
     1081 tctttctaga ggtaccacgc gtcctagtta ttaatagtaa tcaattacgg ggtcattagt
     1141 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     1201 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     1261 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     1321 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     1381 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     1441 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
     1501 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     1561 tgcagcgatg ggggcggggg gggggggggg gcgcgcnnnn nnnnnnctaa attgtaagcg
     1621 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     1681 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     1741 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     1801 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     1861 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     1921 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     1981 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     2041 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     2101 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     2161 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     2221 gtgagcgcgc gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc
     2281 gctgtcgaca taacttcgta tagcatacat tatacgaagt tatctcgacc agcttctgat
     2341 ggaattagaa cttggcaaaa caatactgag aatgaagtgt atgtggaaca gaggctgctg
     2401 atctcgttct tcaggctatg aaactgacac atttggaaac cacagtactt agaaccacaa
     2461 agtgggaatc aagagaaaaa caatgatccc acgagagatc tatagatcta tagatcatga
     2521 gtgggaggaa tgagctggcc cttaatttgg ttttgcttgt ttaaattatg atatccaact
     2581 atgaaacatt atcataaagc aatagtaaag agccttcagt aaagagcagg catttatcta
     2641 atcccacccc acccccaccc ccgtagctcc aatccttcca ttcaaaatgt aggtactctg
     2701 ttctcaccct tcttaacaaa gtatgacagg aaaaacttcc attttagtgg acatctttat
     2761 tgtttaatag atcatcaatt tctgcatccc ggggatctga tatcatcgat gcatggggtc
     2821 gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc
     2881 gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc
     2941 cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc
     3001 cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct
     3061 ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg
     3121 gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca
     3181 tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga
     3241 ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg
     3301 tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt
     3361 ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt
     3421 cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttcagctgct
     3481 cgatcgagat ctagatggat gcaggtcgaa aggcccggag atgaggaaga ggagaacagc
     3541 gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga ccttcgggcg
     3601 cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct tcccagcctc
     3661 tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag gcctacccgc
     3721 ttccattgct cagcggtgct gtccatctgc acgagacttg tgagacgtgc tacttccatt
     3781 tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac taggggagga
     3841 gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct accggtggat
     3901 gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag cggggctgct
     3961 aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta gaatttcgag
     4021 gtcgaagggc gcagtagtcc agggtttcct tgatgatgtc atacttatcc tgtccctttt
     4081 ttttccacag ggatcctcgg ggacaccaaa tatggcgatc tcggcctttt cgtttcttgg
     4141 agctgggaca tgtttgccat cgatccatct accaccagaa cggccgttag atctgctgcc
     4201 accgttgttt ccaccgaaga aaccaccgtt gccgtaacca ccacgacggt tgttgctaaa
     4261 gaagctgcca ccgccacggc caccgttgta gccgccgttg ttgttattgt agttgctcat
     4321 gttatttctg gcacttcttg gttttcctct taagtgagga ggaacataac cattctcgtt
     4381 gttgtcgttg atgcttaaat tttgcacttg ttcgctcagt tcagccataa tatgaaatgc
     4441 ttttcttgtt gttcttacgg aataccactt gccacctatc accacaacta actttttccc
     4501 gttcctccat ctcttttata ttttttttct cgatcgaggg atctttgtga aggaacctta
     4561 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     4621 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     4681 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     4741 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     4801 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     4861 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
     4921 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
     4981 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
     5041 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
     5101 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
     5161 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     5221 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     5281 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5341 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggtcg agggatcttt
     5401 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     5461 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     5521 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     5581 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     5641 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     5701 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     5761 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     5821 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     5881 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     5941 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     6001 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     6061 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     6121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6241 gtcgagggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     6301 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     6361 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     6421 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     6481 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     6541 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     6601 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     6661 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
     6721 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
     6781 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
     6841 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     6901 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     6961 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7021 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7081 tcttatcatg tctggtcgag ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     7141 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     7201 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     7261 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     7321 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     7381 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     7441 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     7501 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     7561 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     7621 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     7681 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     7741 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     7801 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7861 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7921 aactcatcaa tgtatcttat catgtctgga attccgcaag ctagccacca tggcttgagt
     7981 aagtctgcag taagcttcgt atataccttt ctatacgaag ttgtggatct gcgatctaag
     8041 taagcttggc attccggtac tgttggtaaa gccaccatgg aagacgccaa aaacataaag
     8101 aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag
     8161 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag
     8221 gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa
     8281 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc
     8341 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt
     8401 tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt
     8461 tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa
     8521 attattatca tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc
     8581 acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg
     8641 gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc
     8701 gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc
     8761 aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga
     8821 atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt
     8881 gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg
     8941 ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat
     9001 ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc
     9061 aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct
     9121 attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt
     9181 tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc
     9241 gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc
     9301 aacgccttga ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa
     9361 gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag
     9421 gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt
     9481 gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag
     9541 cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc
     9601 gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga
     9661 aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc
     9721 gccgtggagc agctcgagtc catctacagg tactacggcc tgctgctgaa ggaataatct
     9781 agtggcaata aaaagacaga ataagacgca cgggtgttgg gtcgtttgtt ctagaagatg
     9841 ggcgggagtc ttctgggcag gcttaaaggc taacctggtg tgtgggcgtt gtcctgcagg
     9901 ggaattgaac aggtgtaaaa ttggagggac aagacttccc acagattttc ggttttgtcg
     9961 ggaagttttt taataggggc aaataggaaa atggaggata ggagtcatct ggggtttatg
    10021 cagcaaaact acaggtatat tgcttgtatc cgcctcggag atttccatga ggagataaag
    10081 acatgtcacc cgagtttata ctctcctgct tagatcctac tacagtatga aatacagtgt
    10141 ngcgaggtag actatgtaag cagatttaat cattttaaag agcccagtac ttcatatcca
    10201 tttctcccgc tccttctgca gccttatcaa aaggtattta gaacactcat tttagcccca
    10261 ttttcattta ttatactggc ttatccaacc cctagacaga gcattggcat tttccctttc
    10321 ctgatcttag aagtctgatg actcatgaaa ccagacagat tagttacata caccacaaat
    10381 cgaggctgta gctggggcct caacactgca gttcttttat aactccttag tacacttttt
    10441 gttgatcctt tgccttgatc cttaattttc agtgtctatc acctctcccg tcaggtggtg
    10501 ttccacattt gggcctattc tcagtccagg gagttttaca acaatagatg tattgagaat
    10561 ccaacctaaa gcttaacttt ccactcccat gaatgcctct ctcctttttc tccattataa
    10621 ctgagctatn accattaatg gtttcaggtg gatgtctcct cccccaatat acctgatgta
    10681 tctacatatt gccaggctga tattttaaga catnaaaggt atatttcatt attgagccac
    10741 atggtattga ttactgctac taaaattttg tcattgtaca catctgtaaa aggtggttcc
    10801 ttttggaatg caaagttcag gtgtttgttg tctttcctga cctaaggtct tgtgagcttg
    10861 tattttttct atttaagcag tgctttctct tggactggct tgactcatgg cattctacac
    10921 gttattgctg gtctaaatgt gattttgcca agcttcttca ggacctataa ttttgcttga
    10981 cttgtagcca aacacaagta aaatgattaa gcaacaaatg tatttgtgaa gcttggtttt
    11041 taggttgttg tgttgtgtgt gcttgtgctc tataataata ctatccaggg gctggagagg
    11101 tggctcggag ttcaagagca cagactgctc ttccagaagt cctgagttca attcccagca
    11161 accacatggt ggctcacaac catctgtaat gggatctgat gccctcttct ggtgtgtctg
    11221 aagaccacaa gtgtattcac attaaataaa taatcctcct tcttcttctt tttttttttt
    11281 taaagagaat nctgtctcca gtagaattac tgaagtaatg aaatactttg tgtttgttcc
    11341 aatatggnag ccaataatca aatactcttn agcactggaa atgtaccaag gaactatttt
    11401 atttaagtgn actgtggaca gaggagccat aactgcagac ttgtgggata cagaagacca
    11461 atgcagactt aatgtctttt ctcttacact aagcaataaa gaaataaaaa ttgaacttct
    11521 agtatcctat ttgttaaact gctagcttta ctaacttttg tgcttcatct atacaaagct
    11581 gaaagctaag tctgcagcca ttactaaaca tgaaagcaag taatgataat tttggatttc
    11641 aaaaatgtag ggccagagtt tagccagcca gtggtggtgc ttgcctttat gccttaatcc
    11701 cagcactctg gaggcagaga caggcagatc tctgagtttg agcccagcct ggtctacaca
    11761 tcaagttcta tctaggatag ccaggaatac acacagaaac cctgttgggg aggggggctc
    11821 tgagatttca taaaattata attgaagcat tccctaatga gccactatgg atgtggctaa
    11881 atccgtctac ctttctgatg agatttgggt attatttttt ctgtctctgc tgttggttgg
    11941 gtcttttgac actgtgggct ttcttaaagc ctccttccct gccatgtggt ctcttgtttg
    12001 ctactaactt cccatggctt aaatggcatg gctttttgcc ttctaagggc agctgctgag
    12061 ntttgcagcc tgatttccag ggtggggttg ggaaatcttt caaacactaa aattgtcctt
    12121 taattttttt ttaaaaaatg ggttatataa taaacctcat aaaatagtta tgaggagtga
    12181 ggtggactaa tattaatgag tccctcccct ataaaagagc tattaaggct ttttgtctta
    12241 tactaacttt ttttttaaat gtggtatctt tagaaccaag ggtcttagag ttttagtata
    12301 cagaaactgt tgcatcgctt aatcagattt tctagtttca aatccagaga atccaaattc
    12361 ttcacagcca aagtcaaatt aagaatttct gactttaatg ttatttgcta ctgtgaatat
    12421 aaaatgatag cttttcctga ggcagggtct cactatgtat ctctgcctga tctgcaacaa
    12481 gatatgtaga ctaaagttct gcctgctttt gtctcctgaa tactaaggtt aaaatgtagt
    12541 aatacttttg gaacttgcag gtcagattct tttatagggg acacactaag ggagcttggg
    12601 tgatagttgg taaatgtgtt taagtgatga aaacttgaat tattatcacc gcaacctact
    12661 ttttaaaaaa aaaagccagg cctgttagag catgctaagg gatccctagg acttgctgag
    12721 cacacaagag tagtacttgg caggctcctg gtgagagcat atttcaaaaa acaaggcaga
    12781 caaccaagaa actacagtaa ggttacctgt ctttaaccat ctgcatatac acagggatat
    12841 taaaatattc caaataatat ttcattcaag ttttccccca tcaaattggg acatggattt
    12901 ctccggtgaa taggcagagt tggaaactaa acaaatgttg gttttgtgat ttgtgaaatt
    12961 gttttcaagt gatagttaaa gcccatgaga tacagaacaa agctgctatt tcgaggtctc
    13021 ttggttatac tcagaagcac ttctttgggt ttccctgcac tatcctgatc atgtgctagg
    13081 cctnccttag gctgattgtt gttcaaataa cttaagtttc ctgtcaggtg atgtcatatg
    13141 atttcatata tcaaggcaaa acatgttata tatgttaaac atttgnactt aatgtgaaag
    13201 ttaggtcttt gtgggttttg attttaattt caaaacctga gctaaataag tcattttaca
    13261 tgtcttacat ttggtgaatt gtatattgtg gtttgcaggc aagactctct gacctagtaa
    13321 ccctcctata gagcactttg ctgggtcaca agtctaggag tcaagcattt caccttgaag
    13381 ttgagacgtt ttgttagtgt atactagtta tatgttggag gacatgttta tccagaagat
    13441 attcaggact atttttgact gggctaagga attgattctg attagcactg ttagtgagca
    13501 ttgagtggcc tttaggcttg aattggagtc acttgtatat ctcaaataat gctggccttt
    13561 tttnaaaagc ccttgttctt tatcaccctg ttttctacat aatttttgtt caaagaaata
    13621 cttgtttgga tctccttttg acaacaatag catgttttca agccatattt tttttccttt
    13681 tttttttttt ttttggtttt tcgagacagg gtttctctgt atagccctgg ctgtcctgga
    13741 actcactttg tagaccaggc tggcctcgaa ctcagaaatc cgcctgcctc tgcctcctga
    13801 gtgccgggat taaaggcgtg caccaccacg cctggctaag ttggatattt tgtatataac
    13861 tataaccaat actaactcca ctgggtggat ttttaattca gtcagtagtc ttaagtggtc
    13921 tttattggcc cttattaaaa tctactgttc actctaacag aggctgttgg actagtggna
    13981 ctaagcaact tcctacggat atactagcag ataagggtca gggatagaaa ctagtctagc
    14041 gttttgtata cctaccagct tatactacct tgttctgata gaaatattta ggacatctag
    14101 cttatcgatc cgtcgacggt atcgataagc ttgatatcga attctaccgg gtaggggagg
    14161 cgcttttcca aggcagtctg agcatgcgct tagcagcccc gctggcactt ggcgctacac
    14221 aagtggcctn tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
    14281 ctttggtggc cccttcgcgc caccttctnc tcctccccta gtcaggaagt tcccccccgc
    14341 cccgcagctc gcgtcgtnag gacgtgacaa atggaagtag cacgtctcac tagtctcgtc
    14401 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
    14461 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
    14521 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
    14581 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
    14641 cgacctgcag gtcctcgcca tggatcctga tgatgttgtt attcttctaa tcttttgtat
    14701 ggaaaacttt tcttcgtacc acgggactaa acctggttat gtagattcca ttcaaaaagg
    14761 tatacaaaag ccaaaatctg gtacacaagg aaattatgac gatgattgga aagggtttta
    14821 tagtaccgac aataaatacg acgctgcggg atactctgta gataatgaaa acccgctctc
    14881 tggaaaagct ggaggcgtgg tcaaagtgac gtatccagga ctgacgaagg ttctcgcact
    14941 aaaagtggat aatgccgaaa ctattaagaa agagttaggt ttaagtctca ctgaaccgtt
    15001 gatggagcaa gtcggaacgg aagagtttat caaaaggttc ggtgatggtg cttcgcgtgt
    15061 agtgctcagc cttcccttcg ctgaggggag ttctagcgtt gaatatatta ataactggga
    15121 acaggcgaaa gcgttaagcg tagaacttga gattaatttt gaaacccgtg gaaaacgtgg
    15181 ccaagatgcg atgtatgagt atatggctca agcctgtgca ggaaatcgtg tcaggcgatc
    15241 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
    15301 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
    15361 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
    15421 atgcagatcc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
    15481 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
    15541 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
    15601 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
    15661 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg acctcgaggg
    15721 ggggcccggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
    15781 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    15841 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    15901 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    15961 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    16021 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    16081 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    16141 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    16201 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    16261 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    16321 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    16381 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    16441 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    16501 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    16561 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    16621 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    16681 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    16741 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    16801 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    16861 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    16921 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    16981 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    17041 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    17101 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    17161 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    17221 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    17281 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    17341 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    17401 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    17461 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    17521 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    17581 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    17641 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    17701 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    17761 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    17821 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    17881 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
    17941 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
    18001 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
    18061 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
    18121 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
    18181 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
    18241 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
    18301 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
    18361 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
    18421 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    18481 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    18541 gacggccagt gagcgcgcgt aatacgactc actatagggc gaattggagc t
//
