LOCUS       sequence-93481-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mitochondrial Zn-binding FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Park JG, Qin Y, Galati DF, Palmer AE
  TITLE     New Sensors for Quantitative Measurement of Mitochondrial Zn(2+).
  JOURNAL   ACS Chem Biol. 2012 Aug 10.
  PUBMED    22850482
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2012 Aug 10."
COMMENT     Sequence Label: pcDNA-mito-ZifCV1.49
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             56..130
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             152..226
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             248..322
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             344..418
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     complement(494..515)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttatgtc
       61 cgtcctgacg ccgctgctgc tgcggggctt gacaggctcg gcccggcggc tcccagtgcc
      121 gcgcgccaag atccattcgt tgggagatcc tatgtccgtc ctgacgccgc tgctgctgcg
      181 gggcttgaca ggctcggccc ggcggctccc agtgccgcgc gccaagatcc attcgttggg
      241 agatcctatg tccgtcctga cgccgctgct gctgcggggc ttgacaggct cggcccggcg
      301 gctcccagtg ccgcgcgcca agatccattc gttgggagat cctatgtccg tcctgacgcc
      361 gctgctgctg cggggcttga caggctcggc ccggcggctc ccagtgccgc gcgccaagat
      421 ccattcgttg gggaagcttg gggatcccat ggtgagcaag ggcgaggagc tgttcaccgg
      481 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaggt tcagcgtgtc
      541 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      601 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg
      661 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      721 aggctacgtc caggagcgta ccatcttctt caaggacgac ggcaactac
//
