LOCUS       sequence-93482-e         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mitochondrial Zn-binding FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Park JG, Qin Y, Galati DF, Palmer AE
  TITLE     New Sensors for Quantitative Measurement of Mitochondrial Zn(2+).
  JOURNAL   ACS Chem Biol. 2012 Aug 10.
  PUBMED    22850482
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2012 Aug 10."
COMMENT     Sequence Label: pcDNA-mito-ZifCV1.173
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(55..567,568..570,571..573))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     248..267
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     507..528
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(631..652)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tctagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt cttactcgat
       61 gttgtggcgg atcttgaagt tggccttgat gccgttcttc tgcttgtcgg cggtgatata
      121 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
      181 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      241 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
      301 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa
      361 gcactgcagg ccgtagccca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      421 ggtggtgcag atcagcttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
      481 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
      541 cccggtgaac agctcctcgc ccttgctcac catgccaccg ctgccaccct tgtacagctc
      601 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
      661 cttctcgttg gggtctttgc tcagggcgga ctggtagctc aggtagtggt tgtcgggcag
      721 cagcac
//
