LOCUS       sequence-93483-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear zinc-binding FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Miranda JG, Weaver AL, Qin Y, Park JG, Stoddard CI, Lin MZ, Palmer 
            AE
  TITLE     New alternately colored FRET sensors for simultaneous monitoring of 
            Zn(2)(+) in multiple cellular locations.
  JOURNAL   PLoS One. 2012;7(11):e49371. doi: 10.1371/journal.pone.0049371. Epub
            2012 Nov 16.
  PUBMED    23173058
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(11):e49371. doi"; pages: " 
            10.1371/journal.pone.0049371. Epub 2012 Nov 16"
COMMENT     Sequence Label: pcDNA-NLS-ZapCmR1.1
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             99..119
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(183..204)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 ctggctagcg tttaaactta agcttggtac cgagcatgcc taaaaaaaaa cgtaaagttg
      121 aagatgcttc ggatccgatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      181 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg
      241 agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      301 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cgtggcctgc ttcagccgct
      361 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      421 aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt
      481 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      541 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaacgtc tatatcacgg
      601 ccgacaagca gaagaacggc atcaaggcta acttcaagat ccgccacaac gttgaggacg
      661 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      721 tgctgcccga caaccactac ctgagccatc agt
//
