LOCUS       sequence-93485-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytosolic zinc-binding FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Miranda JG, Weaver AL, Qin Y, Park JG, Stoddard CI, Lin MZ, Palmer 
            AE
  TITLE     New alternately colored FRET sensors for simultaneous monitoring of 
            Zn(2)(+) in multiple cellular locations.
  JOURNAL   PLoS One. 2012;7(11):e49371. doi: 10.1371/journal.pone.0049371. Epub
            2012 Nov 16.
  PUBMED    23173058
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(11):e49371. doi"; pages: " 
            10.1371/journal.pone.0049371. Epub 2012 Nov 16"
COMMENT     Sequence Label: pcDNA-NES-ZapCmR1.1
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             97..123
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(178..199)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
       61 cgtttaaact taagcttggt accgagcatg cttcaacttc ctcctcttga acgtcttact
      121 ctttcggatc cgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      181 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tccgcggcga gggcgagggc
      241 gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      301 ccctggccca ccctcgtgac caccttcggc tacggcgtgg cctgcttcag ccgctacccc
      361 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      421 cgcaccatct ctttcaagga cgacggtacc tacaagaccc gcgccgaggt gaagttcgag
      481 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      541 atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat cacggccgac
      601 aagcagaaga acggcatcaa ggctaacttc aagatccgcc acaacgttga ggacggcagc
      661 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
//
