LOCUS       sequence-93487-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytosolic zinc-binding FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Miranda JG, Weaver AL, Qin Y, Park JG, Stoddard CI, Lin MZ, Palmer 
            AE
  TITLE     New alternately colored FRET sensors for simultaneous monitoring of 
            Zn(2)(+) in multiple cellular locations.
  JOURNAL   PLoS One. 2012;7(11):e49371. doi: 10.1371/journal.pone.0049371. Epub
            2012 Nov 16.
  PUBMED    23173058
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(11):e49371. doi"; pages: " 
            10.1371/journal.pone.0049371. Epub 2012 Nov 16"
COMMENT     Sequence Label: pcDNA-NES-ZapCmR2
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..44
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        25..43
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             98..124
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(179..200)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 actgccttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
       61 gcgtttaaac ttaagcttgg taccgagcat gcttcaactt cctcctcttg aacgtcttac
      121 tctttcggat ccgatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      181 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg agggcgaggg
      241 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      301 gccctggccc accctcgtga ccaccttcgg ctacggcgtg gcctgcttca gccgctaccc
      361 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      421 gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg tgaagttcga
      481 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      541 catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata tcacggccga
      601 caagcagaag aacggcatca aggctaactt caagatccgc cacaacgttg aggacggcag
      661 cgtgcagctc gccgaccact accagcagaa ca
//
