LOCUS       sequence-93489-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytosolic zinc-binding FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Qin Y, Miranda JG, Stoddard CI, Dean KM, Galati DF, Palmer AE
  TITLE     Direct comparison of a genetically encoded sensor and small molecule
            indicator: implications for quantification of cytosolic Zn(2+).
  JOURNAL   ACS Chem Biol. 2013 Nov 15;8(11):2366-71. doi: 10.1021/cb4003859. 
            Epub 2013 Sep 3.
  PUBMED    23992616
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb4003859";
            journalName: "ACS Chem Biol"; date: "2013-11-15- 15"; volume: "8"; 
            issue: "11"; pages: "2366-71"
COMMENT     Sequence Label: pcDNA-NES-ZapCY2
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             97..123
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(133..135,136..138,139..651)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(178..199)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(439..458)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
       61 cgtttaaact taagcttggt accgagcatg cttcaacttc ctcctcttga acgtcttact
      121 ctttcggatc ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      181 gtcgagctgg acggcgacgt aaacggccac aggttcagcg tgtccggcga gggcgagggc
      241 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      301 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      361 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      421 cgtaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      481 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      541 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
      601 aagcagaaga acggcatcaa ggcccacttc aagatccgcc acaacatcga ggacggcagc
      661 gtgcagctcg ccgaccacta ccagcagaac a
//
