LOCUS       sequence-93493-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown of human FOXH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, 
            Nakamura M, Sutou K, Osafune K, Yamanaka S
  TITLE     Induction of pluripotency in human somatic cells via a transient 
            state resembling primitive streak-like mesendoderm.
  JOURNAL   Nat Commun. 2014 Apr 24;5:3678. doi: 10.1038/ncomms4678.
  PUBMED    24759836
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-04-24"; volume: "5:3678. doi"; pages: " 
            10.1038/ncomms4678"
COMMENT     Sequence Label: pMKO.1-puro/hFOXH1 shRNA3
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..17
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        69..317
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     69..89
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     240..259
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(402..422)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        407..736
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      587..722
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(746..765)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ctttatccag ccctcactcc ttctctaggc gccggaattg aagatctgtc gacaaggtcg
       61 ggcaggaaga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
      121 ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt
      181 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
      241 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
      301 ggaaaggacg aaacaccggg cctatctaca ctcccaatgt ctcgagacat tgggagtgta
      361 gataggcttt ttgaattcgc cagcacagtg gtcgaccctg tggaatgtgt gtcagttagg
      421 gtgtggaaag tccccaggct tcccagcagg cagaagtatg caaagcatgc atctcaatta
      481 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      541 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
      601 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
      661 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
      721 cctaggcttt tgcaaaaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
      781 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg a
//
