LOCUS       sequence-93494-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to
            drive short RNAs, pgkneo, cassette flanked by loxP sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Brown JR, Zetsche B, Jackson-Grusby L
  TITLE     RUSH and CRUSH: a rapid and conditional RNA interference method in 
            mice.
  JOURNAL   Genesis. 2014 Jan;52(1):39-48. doi: 10.1002/dvg.22718. Epub 2013 Oct
            26.
  PUBMED    24166816
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22718";
            journalName: "Genesis"; date: "2014-01"; volume: "52"; issue: "1"; 
            pages: "39-48"
COMMENT     Sequence Label: pRUSH
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(108..141)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     145..165
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        257..756
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(292..315)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     689..708
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 tgcatataat atttcctagt aactatagag gcttaatgtg cgataaaaga cagataatct
       61 gttcttttta atactagcta cattttacat gataggcttg gatttctata acttcgtata
      121 gcatacatta tacgaagtta taaacagcac aaaaggaaac tcaccctaac tgtaaagtaa
      181 ttgtgtgttt tgagactata aatatccctt ggagaaaagc cttgttaacg cgcggtgacc
      241 ctcgaggaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
      301 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
      361 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
      421 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
      481 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
      541 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
      601 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
      661 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
      721 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagccaata tgggatcggc
      781 cattg
//
