LOCUS       sequence-93495-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox 
            conditional U6 promoter pgk-puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Brown JR, Zetsche B, Jackson-Grusby L
  TITLE     RUSH and CRUSH: a rapid and conditional RNA interference method in 
            mice.
  JOURNAL   Genesis. 2014 Jan;52(1):39-48. doi: 10.1002/dvg.22718. Epub 2013 Oct
            26.
  PUBMED    24166816
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22718";
            journalName: "Genesis"; date: "2014-01"; volume: "52"; issue: "1"; 
            pages: "39-48"
COMMENT     Sequence Label: pCRUSH
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     116..136
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    complement(154..187)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     complement(264..287)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     661..680
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct acattttaca
       61 tgataggctt ggatttctat aagagataca aatactaaat tattatttta aaaaacagca
      121 caaaaggaaa ctcaccctaa ctgtaaagta attataactt cgtatagtat aaattatacg
      181 aagttataag ccttggtttt ttgaattcgt cgacctcgaa attctaccgg gtaggggagg
      241 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
      301 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
      361 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
      421 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
      481 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
      541 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
      601 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
      661 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
      721 g
//
