LOCUS       sequence-93497-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically encoded voltage sensor ArcLight with improved kinetics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Han Z, Jin L, Platisa J, Cohen LB, Baker BJ, Pieribone VA
  TITLE     Fluorescent protein voltage probes derived from ArcLight that 
            respond to membrane voltage changes with fast kinetics.
  JOURNAL   PLoS One. 2013 Nov 27;8(11):e81295. doi: 
            10.1371/journal.pone.0081295. eCollection 2013.
  PUBMED    24312287
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-11-27"; volume: "8(11):e81295. doi"; pages: " 
            10.1371/journal.pone.0081295. eCollection 2013"
COMMENT     Sequence Label: Chicken ArcLight A173
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(634..662)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagct agcttgattt aggtgacact atagaataca
       61 agcttcgcca ccatggagac gactgtgagg tatgaacagg ggtcagagct cactaaaact
      121 tcgagctctc caacagcaga tgagcccacg ataaagattg atgatggtcg tgatgagggt
      181 aatgaacaag acagctgttc caataccatt aggagaaaaa tttccccgtt tgtgatgtca
      241 tttggattca gagtatttgg agttgtgctt atcattgtag acatcatagt ggtgattgtg
      301 gatctggcca tcagtgagaa gaaaagaggc attagagaga ttcttgaagg tgtttccctg
      361 gctatagcac tcttcttcct tgttgatgtt ctcatgagag tgtttgttga aggcttcaag
      421 aactatttcc ggtccaaact gaatactttg gatgcagtca tagtagtggg cactctgcta
      481 attaatatga cctactcctt ctctgacctt gctgccacag atcagatgcc gcagatggtt
      541 actcttcttc gagttctgag aattgttatc ttaataagaa tatttcgcct ggctggggat
      601 cccatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      661 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      721 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      781 cttgtcacta ctttaactta tggtgttcaa tgcttttcaa gatacccaga tcatatgaa
//
