LOCUS       sequence-93500-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression Vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Hopkins BD, Fine B, Steinbach N, Dendy M, Rapp Z, Shaw J, Pappas K, 
            Yu JS, Hodakoski C, Mense S, Klein J, Pegno S, Sulis ML, Goldstein 
            H, Amendolara B, Lei L, Maurer M, Bruce J, Canoll P, Hibshoosh H, 
            Parsons R
  TITLE     A secreted PTEN phosphatase that enters cells to alter signaling and
            survival.
  JOURNAL   Science. 2013 Jul 26;341(6144):399-402. doi: 
            10.1126/science.1234907. Epub 2013 Jun 6.
  PUBMED    23744781
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1234907"; journalName: "Science"; date: 
            "2013-07-26- 26"; volume: "341"; issue: "6144"; pages: "399-402"
COMMENT     Sequence Label: JpExpress404 PTEN-LONG
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    203..219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(244..258,259..264,265..281,282..288)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    264..280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    294..313
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga gaattaagaa
       61 aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt atacatgtca
      121 acgataatac aaaatataat acaaactata agatgttatc agtatttatt atcatttaga
      181 ataaattttg tgtcgccctt aattgtgagc ggataacaat tacgagcttc atgcacagtg
      241 aaatcatgaa aaatttattt gctttgtgag cggataacaa ttataatatg tggaattgtg
      301 agcgctcaca attccacaac ggtttccctc tagaaataat tttgtttaac ttttaggagg
      361 taaaacatat gagcgagtcc ccagtcacca tcagcagagc aggcaacgca ggtgaactgg
      421 ttagcccgtt gctgctgccg ccgacgcgcc gtcgtcgtcg ccgtcacatt cagggtcctg
      481 gtccggtgct gaacctgccg tctgctgcag ctgctccgcc ggttgctcgt gcgccagagg
      541 cagcgggtgg cggttcccgt tccgaggatt atagctcctc gccgcatagc gcagcagcgg
      601 cagctcgtcc gctggcggca gaggaaaagc aggctcagtc gctgcaaccg agctctagcc
      661 gtcgttctag ccactaccca gcggccgttc agagccaggc agccgcagaa cgtggcgcga
      721 gcgcgacggc caaaagccgc gcaattagca ttctgcaaaa gaaaccgcgt caccaacaac
      781 tgttgccaag cttgagcagc ttctttt
//
