LOCUS       sequence-93503-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression Vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Hopkins BD, Fine B, Steinbach N, Dendy M, Rapp Z, Shaw J, Pappas K, 
            Yu JS, Hodakoski C, Mense S, Klein J, Pegno S, Sulis ML, Goldstein 
            H, Amendolara B, Lei L, Maurer M, Bruce J, Canoll P, Hibshoosh H, 
            Parsons R
  TITLE     A secreted PTEN phosphatase that enters cells to alter signaling and
            survival.
  JOURNAL   Science. 2013 Jul 26;341(6144):399-402. doi: 
            10.1126/science.1234907. Epub 2013 Jun 6.
  PUBMED    23744781
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1234907"; journalName: "Science"; date: 
            "2013-07-26- 26"; volume: "341"; issue: "6144"; pages: "399-402"
COMMENT     Sequence Label: JpExpress404 PTEN
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    200..216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(241..255,256..261,262..278,279..285)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    261..277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    291..310
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 attatttatg attttttgta tatacaatat ttctagtttg ttaaagagaa ttaagaaaat
       61 aaatctcgaa aataataaag ggaaaatcag tttttgatat caaaattata catgtcaacg
      121 ataatacaaa atataataca aactataaga tgttatcagt atttattatc atttagaata
      181 aattttgtgt cgcccttaat tgtgagcgga taacaattac gagcttcatg cacagtgaaa
      241 tcatgaaaaa tttatttgct ttgtgagcgg ataacaatta taatatgtgg aattgtgagc
      301 gctcacaatt ccacaacggt ttccctctag aaataatttt gtttaacttt taggaggtaa
      361 aacatatgac cgcgattatc aaagagatcg tgtcccgtaa caagcgccgt tatcaagaag
      421 atggctttga cctggacctg acctacatct acccgaacat tatcgcgatg ggttttccag
      481 ccgagcgcct ggagggcgtc taccgtaata acatcgatga tgtggttcgt tttctggaca
      541 gcaaacataa gaaccattac aagatctata atctgtgcgc cgagcgtcat tatgacaccg
      601 cgaaattcaa ttgccgtgtt gcgcagtacc cgttcgagga ccacaatccg ccgcaactgg
      661 aactgattaa accgttctgc gaggacctgg atcagtggct gtccgaggac gacaaccacg
      721 tggccgcgat tcactgtaag gccggcaaag gtcgtaccgg cgtcatgatc tgcgcgtacc
      781 tgctgcaccg cggtaagttt ctgaaggcgc aagaagcgct ggactt
//
