LOCUS       sequence-93505-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically encoded voltage sensor ArcLight with improved kinetics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Han Z, Jin L, Platisa J, Cohen LB, Baker BJ, Pieribone VA
  TITLE     Fluorescent protein voltage probes derived from ArcLight that 
            respond to membrane voltage changes with fast kinetics.
  JOURNAL   PLoS One. 2013 Nov 27;8(11):e81295. doi: 
            10.1371/journal.pone.0081295. eCollection 2013.
  PUBMED    24312287
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-11-27"; volume: "8(11):e81295. doi"; pages: " 
            10.1371/journal.pone.0081295. eCollection 2013"
COMMENT     Sequence Label: Chicken ArcLight S174
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        42..60
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     42..59
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(642..670)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agggaaccgc cattctgcct ggggacgtcg gagctagctt gatttaggtg acactataga
       61 atacaagctt cgccaccatg gagacgactg tgaggtatga acaggggtca gagctcacta
      121 aaacttcgag ctctccaaca gcagatgagc ccacgataaa gattgatgat ggtcgtgatg
      181 agggtaatga acaagacagc tgttccaata ccattaggag aaaaatttcc ccgtttgtga
      241 tgtcatttgg attcagagta tttggagttg tgcttatcat tgtagacatc atagtggtga
      301 ttgtggatct ggccatcagt gagaagaaaa gaggcattag agagattctt gaaggtgttt
      361 ccctggctat agcactcttc ttccttgttg atgttctcat gagagtgttt gttgaaggct
      421 tcaagaacta tttccggtcc aaactgaata ctttggatgc agtcatagta gtgggcactc
      481 tgctaattaa tatgacctac tccttctctg accttgctgc cacagatcag atgccgcaga
      541 tggttactct tcttcgagtt ctgagaattg ttatcttaat aagaatattt cgcctggctt
      601 caggggatcc catgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      661 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      721 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaa
//
