LOCUS       sequence-93512-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral GFP reporter for testing enhancer activity in different 
            cell types. Insert is an enhancer identified in the exonic region of
            the keratin type II cluster. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  TITLE     Yu Lab-keratin enhancer GFP reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Enh eGFP Reporter-muKC2-X5
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..110
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             386..403
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctcacccgaa tgacatatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
       61 tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagcaggct ttggatgcct
      121 ccaggtgggc acaccgggaa gccagctccc ccatagccac caccaaagcc agctccacca
      181 ccgaagccaa agccactacc agctccacca ccaaaaccaa atccactgcc ggctcctcct
      241 ccaaagccaa agccaccagc accaaattgg ttcctgaagc tgcctccgcc agaactgtag
      301 gatatccttt tggagccccc cacattgtac aggctccggc tgccatagcc cccggctcca
      361 caagcacccc caaggctgat cctgccacca ccaccaccac cactgcggga caccgagctg
      421 aagctcgtac gagagacaga tggggtgatg gcagaggcgg cgctgaaaga gcggctgccc
      481 ccacttcgga aggacacact ggactggcga gacatgatgg ttcctggagg agcaagaaca
      541 ctgggcgctg gtggtgggga ggtgctggcg aggtcagcgg cgttcaacag gacgctgtgg
      601 gtggctctgt aacttaggag atttgaggtc cccttttatc cccttagaag tgggttgggc
      661 agaggagctg tcgagctgtg aatgattagt gggctgggca tgcctggggg gcagctgtga
      721 gctcacccag ctcacctgca cagtggtgtg gggtgtaaat gctgctccca gcaggctctg
      781 ctcagttagg aataaccttt ccttacagag ctggtgtcct gtcagtgtgt tgggggggtt
      841 ctctcgtcac cccagatctg ggctttgtgt gtagcctaca agatgccagg acattacggg
      901 ctatacccac ctttctt
//
