LOCUS       sequence-93514-e        1281 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral GFP reporter for testing enhancer activity in different 
            cell types. Insert is an enhancer identified in the exonic region of
            the keratin type II cluster. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1281)
  TITLE     Yu Lab-keratin enhancer GFP reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Enh eGFP Reporter-muKC2-X7
FEATURES             Location/Qualifiers
     source          1..1281
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..106
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(766..865)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(882..901)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(883..901)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(907..923)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(907..923)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tcnactgaca atatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
       61 gagcaatgct tttttataat gccaactttg tacaaaaaag caggctttat tgctatggtc
      121 tggaatcctg gacagcccta tgcaggtgca tcttctctct cagggatatt cactcaagca
      181 agcacctacc ttggtctggt accaggactc agcctctgtt cggctgcggt tggcaatgtc
      241 ctcgtactgg gccttgacct cagcgatgat gctgtccagg tccaggctgc ggttgttgtc
      301 catggaaagg accacagatg tgtcagagac gtgtgtctgc atctgggaca gctcctacag
      361 ggacccttga agttagtcat ctgattcttt tatttaactt gagtttcatt caaacttccc
      421 atctattgtg tcataataaa atatccacag ggaaaatttg ggttaattgc tcaccgcatc
      481 aaagaacatc ttcatgaagt tgatctcgtc catcagggca tcgaccctgg cctccagctc
      541 caccttgttc atgtaggcag catccacatc ctaaggaaca cagatggttg gcatggaccg
      601 acatgcacct acctcaaatg gctccgcctc caggagagca cagcccaggg ctgctgcttt
      661 cccttcgcca cacccacctt cttcaacatc acaaactcat tctcagccgt ggtacgcttg
      721 ttgatctcat cctcatacct ggtggtcaaa gggacaggaa ggataaccca gctttcttgt
      781 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
      841 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
      901 acatgggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaattct ctgatgttac
      961 attgcacaag ataaaaatat atcatcatga acaataaact gtctgcttac ataaacagta
     1021 atacaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taattccaca
     1081 tgaatgctga ttttaatggg tatatgggct cgcgataatg tcggcatcag gtgcgaacat
     1141 tctatcgctt gtatggagcc gatggcccag agttgtttct ggaacatgca aggtagcgtg
     1201 caatgatgtt nnatgaatgc nacttactgc ctgacgaatt ttatggcccc tntccgacan
     1261 nncgcaacgt cacttntcnc t
//
