LOCUS       sequence-93515-e        1243 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral GFP reporter for testing enhancer activity in different 
            cell types. Insert is an enhancer identified in the exonic region of
            the keratin type II cluster. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1243)
  TITLE     Yu Lab-keratin enhancer GFP reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Enh eGFP Reporter-muKC2-X8
FEATURES             Location/Qualifiers
     source          1..1243
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(931..1030)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1071..1087)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1071..1087)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacgagacaa caaagcatat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagcagg ctttaagctg
      121 gagtgagcac cagcacatat gaatgagtgc agagcagggt ttgcctccta ggatcttgca
      181 ttccatgtaa gacctcaagc cagaagggga tgcctcgcag ccaccattgc ctgtgccaga
      241 agagtgaaga acatgcagta gctggatgga gagcccagag gttgtgagac caggtcagca
      301 agagcttccc cgagaaagat gatccaggag aacgaggaag atgggttggg gtgagggtaa
      361 aggtgggtgt gatggagacg ccaggtctgg aaaagaatgt gggcaaaagc agacagggga
      421 cacggtgcag gaagcatctt agttatttga ggaggacagc atccaagcac tgaaatgggc
      481 agtaagccaa agacgccagt caaggcccgc agtatgccca catgtcagaa tgaaacagga
      541 ggacattcag ccatttgcgt gtgtatctat catccctcat caaactctac attctggagg
      601 ccacctcaag cacactacgg ccctcacctg cactcctcgc cctccagcag cttcctgtag
      661 gtggcgatct ccacgtccag agccagcttg gtgttcatga gctcctggta ctcccgcagc
      721 agcctggcca tgtcctgctt ggccttctgc agggcctcct ccagctctgt cagcttgttt
      781 ctggcatctt tgagagccag ctccccgcgt tgctcagctt cagcaatggc gttctggagg
      841 ttggcacact gcagagagaa agggagcaat ggggggcacg tgggtagctg aactgaccca
      901 cactatcctt ttctgagatc ctaagtcaga acccagcttt cttgtacaaa gttggcatta
      961 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     1021 tcattatttg ccatccagct gattatcccg tatagtgagt cgtttacatg gtcatagctg
     1081 tttcctggca gcttctggcc cgtgttctta aatcttctga tgtacacatt gcnaagataa
     1141 anatatcatc atggacaata aactgtcctg ctactaacag taaataacag gngtntatgn
     1201 ccatattcac ggnaacgtcn nncntcgcat ataaattcca cac
//
