LOCUS       sequence-93529-e        1193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral GFP reporter for testing enhancer activity in different 
            cell types. Insert is an enhancer identified in the exonic region of
            the keratin type II cluster. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1193)
  TITLE     Yu Lab-keratin enhancer GFP reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Enh eGFP Reporter-muKC2-X20
FEATURES             Location/Qualifiers
     source          1..1193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(697..796)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(837..853)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(837..853)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1033..1052)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtac aaaaaagcag gctttcaatc tatcatgaga tgtggaccgg
      121 gtacgtcctt ctgagcaccg tatacttccc gggcaacaca ctggtgtgtg cttagacttg
      181 tgtttaagct agatttccct ggtgtcagaa ctccacttgt gcagtttccg tgagggatgt
      241 gaaggagtgc agcctcagag tactcaccct gagcgcagtg tctcaccttc gtctggtacc
      301 aggactcagc ctctgcccgg cttcggttgg caatgtcctc gtactgggcc ttgacctcgg
      361 cgatgatgct gtccaggtcc aggctgcggt tgttgtccat ggagaggacc acagaggtgt
      421 cactcacctg gttctgcatc tgagacagct cctgcagggt tgtcaactca gagtcaccca
      481 ggaacaaaag gaagggacct tgcacttctg aggtaactaa ctacttaggc taggtctctc
      541 ttctctggca tccctggcag aagtccttcc aggcgcccct cccctccccc agtcctgtta
      601 atcatgtccc cgggagttcc tagacgactc atcagtgtgg tggtgggacc tgttctctcc
      661 agttccagtg atgctttcca ctggtgacac aatcaaaccc agctttcttg tacaaagttg
      721 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
      781 ataaaatcat tatttgccat ccagctgata tcccctatag ngagtcgtat tacatggtca
      841 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
      901 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacanggg
      961 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg
     1021 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gnaatcngng cgacnatcnn
     1081 ncgcttgnnn nggnnngncn nangcgccnn nnnngnttnn tgaannatgn nnaangnanc
     1141 ntnncnaann nannnttnnn nnnaannnan nnnngnncan annnnnnaac nng
//
