LOCUS       sequence-93550-e        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral GFP reporter for testing enhancer activity in different 
            cell types. Insert is an enhancer identified in the exonic region of
            the keratin type II cluster. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  TITLE     Yu Lab-keratin enhancer GFP reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Enh eGFP Reporter-muKC2-X40
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(693..792)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(833..849)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(833..849)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(951..970)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
       61 aatgccaact ttgtacaaaa aagcaggctt tgtaagcctg cacggtacct tatcaatgaa
      121 ggaggcaaat ttgttgttga gggtcttgat ctgctccttt tcgtctctct tcaccctctg
      181 ggcattgggg tcaatctcca ggttgagggg tgtcagcagg ctctggttaa cagttacagc
      241 tgtgatagcg ggggctgatg ggcctccaat tcctccgatt ctgtagccaa agccagggcc
      301 accaaagcca gggcctccga atccagggcc tccgaatcca tagctagggc agccgccagc
      361 cccaaacccc aggccaacac cgctgctggc cctaaagccc aggccatcac cagatccaaa
      421 ggccatccca ctgccagctc caaagccaac tccacagcgg acaggacgag agcatccaac
      481 tgctatccgg ggtgaggatg atccaaagtt gatgacgctc cgactaccaa aaccacccaa
      541 gccccggaag ccaggcccac tcctgcagga gacagagctg gaccggaagc ggttcagatg
      601 ctgaggggcc attgctgagc aagaactgaa gcttcccacc cgacggccag agctgactct
      661 gtaggagcgg caagacatgg tggctttgta gaacccagct ttcttgtaca aagttggcat
      721 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
      781 aatcattatt tgccatccag ctgatatccc ctatagngag tcgtattaca tggtcatagc
      841 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
      901 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
      961 tatgagccat attcaacngn aaacgtcgag gccgcgatta aatncnacat ggnngctgat
     1021 ttannntggg nannnaatgg gctcnnnann aatgtcgggg caatcnnggn ngcnannann
     1081 cnnantcgct
//
