LOCUS       sequence-93551-e        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral GFP reporter for testing enhancer activity in different 
            cell types. Insert is an enhancer identified in the exonic region of
            the keratin type II cluster. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  TITLE     Yu Lab-keratin enhancer GFP reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Enh eGFP Reporter-muKC2-X41
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(698..797)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(814..833)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(815..833)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(838..854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(838..854)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgatnnnat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctttacct gagccttggt atgctcgatc
      121 tctgctttca gcctctggat caaccgggtc agttcattga tctcgtcccg tgtgctacgt
      181 agattgtcac agtgctggcc agcggtcacc cgcatctcct catactgggg gtggaggtgg
      241 ggtggggtgg ggcaaagttt tgaccataca tagtctgggc tgaagacctc agaccaaaat
      301 cttcaccttt ggacagggca cctgtacccc tcccacagtc tcccattgcc caccttggtc
      361 tggtaccacg actccacatc tgcacgactc cgcctcgcca cctcctcata ctgtgccttg
      421 acttcagcta tgatcccatc taggttcaga tcccggctgt tgtccatctt cacgatgaca
      481 gatgtctctg atatgtgtga ttgcagcatc tcgatttcct gtattggaaa agcagtaggg
      541 cagaggggct cagtggtcca tcagcagctg ggactctgcc acagggtccc ctgtgaatca
      601 gggacaggga gcattgcttc tcgctttatg actgtgagag tccccattcc tacctggcct
      661 ggggctcaga aaaaggcatg cttgagaagc aattaaaacc cagctttctt gtacaaagtt
      721 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
      781 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
      841 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
      901 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
      961 ggngnnatga gccatattca acgggaaacg tcganggccg cgattaaatt ccaacatggn
     1021 tgctgattta tatgggtata aatgggctcn nnatnatgtc ggnaatcagg tgcgacaatc
     1081 tnntngctnn nannggaanc ccgatgnnnc cnannant
//
