LOCUS       sequence-93552-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His
            tag) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Maclaurin D, Venkatachalam V, Lee H, Cohen AE
  TITLE     Mechanism of voltage-sensitive fluorescence in a microbial 
            rhodopsin.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44. doi: 
            10.1073/pnas.1215595110. Epub 2013 Mar 25.
  PUBMED    23530193
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1215595110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-9- 9"; volume: "110"; issue: "15"; pages: "5939-44"
COMMENT     Sequence Label: VV020: WT Arch-3 - eGFP in pET28b
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tggaccccat cgctctgcag gctggttacg acctgctggg
      181 tgacggcaga cctgaaactc tgtggctggg catcggcact ctgctgatgc tgattggaac
      241 cttctacttt ctggtccgcg gatggggagt caccgataag gatgcccggg aatattacgc
      301 tgtgactatc ctggtgcccg gaatcgcatc cgccgcatat ctgtctatgt tctttggtat
      361 cgggcttact gaggtgaccg tcgggggcga aatgttggat atctattatg ccaggtacgc
      421 cgactggctg tttaccaccc cacttctgct gctggatctg gcccttctcg ctaaggtgga
      481 tcgggtgacc atcggcaccc tggtgggtgt ggacgccctg atgatcgtca ctggcctcat
      541 cggagccttg agccacacgg ccatagccag atacagttgg tggttgttct ctacaatttg
      601 catgatagtg gtgctctatt ttctggctac atccctgcga tctgctgcaa aggagcgggg
      661 ccccgaggtg gcatctacct ttaacaccct gacagctctg gtcttggtgc tgtggaccgc
      721 ttaccctatc ctgtggatca taggcactga gggcgctggc gtggtgg
//
