LOCUS       sequence-93577-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 530, Emission:
            541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-pBAD
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..88
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..124
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(128..136,137..151)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             167..880
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
       61 ggggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
      121 gggtcgggat ctgtacgacg atgacgataa ggatccgagc tcgagcatgg tgagcaaggg
      181 cgaggggcaa tcgaagcatg ggctaaagga ggagatgacg gtgaagtacc acatggaagg
      241 ttgcgttaat ggtcacaaat ttgtcattac tggagagggc attggaaacc cttttaaggg
      301 taagcaaact gcaaatttgt gtgtgataga aggaggcccg ctgccgttct cggaggacat
      361 tttaagtccg ggttttaaat atggtgaccg gattttcaca gagtacccac aggatattgt
      421 agattacttc aagaactcat gtccagcggg ctatacgtgg gaaaggagct tcctctttga
      481 ggacggagcg gtctgtcgat gcaacgtgga cataacagtc tctgaaaagg agaactgcat
      541 ctatcacaaa agtatcttca gaggggtgaa ttttcccgcc gacggccccg taatgaagaa
      601 aatgaccact aattgggaag ctagtaccga gaaaattgtg cctgttccaa agcaagggat
      661 attaaaagga aaggtcaaaa tgtacctatt gctgaaggat ggcggtcgtt atcattgcca
      721 gtttgatacg gtatataaag ctaaatcagt gccctccaag atgccagaat ggcattttat
      781 acagcataag ctccttaggg aggaccgctc tgatgctaaa aaccagaaat ggcaattaac
      841 agaacatgca atcgcaggca tggacgagct gtacaagtaa gaattcgaag cttggctgtt
//
