LOCUS       sequence-93579-e         328 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of human SRF with an HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Johansen FE, Prywes R
  TITLE     Identification of transcriptional activation and inhibitory domains 
            in serum response factor (SRF) by using GAL4-SRF constructs.
  JOURNAL   Mol Cell Biol. 1993 Aug . 13(8):4640-7.
  PUBMED    8336707
REFERENCE   2  (bases 1 to 328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1993 Aug . 13(8):4640-7."
COMMENT     Sequence Label: pCGN-SRF
FEATURES             Location/Qualifiers
     source          1..328
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           106..145
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             175..201
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             181..207
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgggggatct tggtggcgtg
      121 aaactcccgc acctcttcgg ccagcgcctt gtagaagcgc gtatggcttc tagctatcct
      181 tatgacgtgc ctgactatgc cagcctggga ggaccttcta gagctatgtt accgacccaa
      241 gctggggccg cggcggctct gggccggggc tcggccctgg ggggcagcct gaaccggacc
      301 ccgacggggc ggccgggcgg cggcggcg
//
