LOCUS       sequence-93582-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 514, Emission:
            527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mTopaz-pBAD
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             47..69
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             89..106
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             110..142
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             155..175
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(182..184,185..187,188..901)
                     /codon_start=1
                     /product="enhanced yellow variant of GFP"
                     /label=Topaz YFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(227..248)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(488..507)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     835..856
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 actctctact gtttctccat acccgttttt ttgggctaga aataattttg tttaacttta
       61 agaaggagat atacatatgc ggggttctca tcatcatcat catcatggta tggctagcat
      121 gactggtgga cagcaaatgg gtcgggatct gtacgagaac ctgtacttcc agggctcgag
      181 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      241 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      301 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      361 cctcgtgacc accttcggct acggcgtgca gtgcttcgcc cgctaccccg accacatgcg
      421 ccagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      481 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      541 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      601 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      661 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      721 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      781 ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
      841 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
      901 a
//
