LOCUS       sequence-93586-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 554, Emission:
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien 
            RY
  TITLE     Improved monomeric red, orange and yellow fluorescent proteins 
            derived from Discosoma sp. red fluorescent protein.
  JOURNAL   Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21.
  PUBMED    15558047
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol."; date: "2004-12"; volume: "22(12)"; pages: "1567-72. 
            Epub 2004 Nov 21"
COMMENT     Sequence Label: tdTomato-pBAD
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             52..74
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             94..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..147
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             160..180
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(320..338)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     588..607
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa
       61 ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca tggtatggct
      121 agcatgactg gtggacagca aatgggtcgg gatctgtacg agaacctgta cttccagggc
      181 tcgagcatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
      241 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      301 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
      361 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
      421 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      481 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
      541 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
      601 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
      661 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
      721 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
      781 aagctggaca tcacctcc
//
