LOCUS       sequence-93588-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 553, Emission:
            574.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, 
            Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, 
            Chudakov DM
  TITLE     Bright monomeric red fluorescent protein with an extended 
            fluorescence lifetime.
  JOURNAL   Nat Methods. 2007 Jul;4(7):555-7. Epub 2007 Jun 17.
  PUBMED    17572680
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2007-07"; volume: "4(7)"; pages: "555-7. Epub 2007
            Jun 17"
COMMENT     Sequence Label: TurboRFP-pBAD
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             52..74
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             94..111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..147
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             160..180
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa
       61 ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca tggtatggct
      121 agcatgactg gtggacagca aatgggtcgg gatctgtacg agaacctgta cttccagggc
      181 tcgagcatga gcgagctgat caaggagaac atgcacatga agctgtacat ggagggcacc
      241 gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
      301 cagaccatga agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
      361 gctaccagct tcatgtacgg cagcaaagcc ttcatcaacc acacccaggg catccccgac
      421 ttctttaagc agtccttccc tgagggcttc acatgggaga gaatcaccac atacgaagac
      481 gggggcgtgc tgaccgctac ccaggacacc agcttccaga acggctgcat catctacaac
      541 gtcaagatca acggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacacgc
      601 ggctgggagg ccaacaccga gatgctgtac cccgctgacg gcggcctgag aggccacagc
      661 cagatggccc tgaagctcgt gggcgggggc tacctgcact gctccttcaa gaccacatac
      721 agatccaaga aacccgctaa gaacctcaag atgcccggct tccacttcgt ggaccacaga
      781 ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga gatggctgtg
      841 gccaagtact gcgacctccc tagcaa
//
