LOCUS       sequence-9359-ex       10656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO
  TITLE     A system for Cre-regulated RNA interference in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.
  PUBMED    18779577
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Sep 16. 105(37):13895-900."
COMMENT     Sequence Label: pLB2 CAG P2Gm
FEATURES             Location/Qualifiers
     source          1..10656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    489..510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(525..530,531..548,549..555)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    563..579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     568..590
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     587..603
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     587..603
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             869..1049
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1096..1221
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1720..1953
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2138..2182
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2480..2597
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3119..3422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3424..3701
                     /label=chicken beta-actin promoter
     intron          3703..4720
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4644..4666
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4728..4747
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4809..4818
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4815..5411
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5311..5331
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5433..5498
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     regulatory      5508..5517
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5514..5516,5517..5519,5520..6233)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5514..5516,5517..5519,5520..6230)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5559..5580)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5820..5839)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6167..6188
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           6283..6404
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           6431..6445
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           6473..6599
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    6685..7273
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6738..6758)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7156..7167)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7276..7292)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7277..7293)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    8436..8643
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     LTR             8662..8842
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8904..9492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8984..9003)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9663..10454,10455..10523))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10286..10305
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10524..10628)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagtcat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      301 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
      361 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
      421 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      481 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      541 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
      601 gaccatgatt acgccaagcg cgcaattaac cctagcttaa tgtagtctta tgcaatactc
      661 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
      721 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
      781 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
      841 tttaagtgcc tagctcgata caataaacgg gtctctctgg ttagaccaga tctgagcctg
      901 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      961 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1021 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     1081 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     1141 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     1201 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     1261 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     1321 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1381 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1441 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1501 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1561 gccggccgcg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1621 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1681 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1741 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1801 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1861 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1921 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1981 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     2041 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     2101 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     2161 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     2221 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     2281 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     2341 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2401 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa
     2461 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     2521 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2581 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggttagtac
     2641 cgggcccggt gctttgctct gagccagccc accagtttgg aatgactcct ttttatgact
     2701 tgaattttca agtataaagt ctagtgctaa atttaatttg aacaactgta tagtttttgc
     2761 tggttggggg aaggaaaaaa aatggtggca gtgttttttt cagaattaga agtgaaatga
     2821 aaacttgttg tgtgtgagga tttctaatga catgtggtgg ttgcatactg agtgaagccg
     2881 gtgagcattc tgccatgtca ccccctcgtg ctcagtaatg tactttacag aaatcctaaa
     2941 ctcaaaagat tgatataaac catgcttctt gtgtatatcc ggtctcttct ctgggtagtc
     3001 tcactcagcc tgcatttctg ccagggcccg ctctagccgt taattaactg caggaatcta
     3061 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     3121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     3181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     3241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     3301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     3361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     3421 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
     3481 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
     3541 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
     3601 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
     3661 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc ggggagtcgc tgcgacgctg
     3721 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
     3781 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     3841 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
     3901 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     3961 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     4021 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     4081 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     4141 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     4201 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     4261 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     4321 ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     4381 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     4441 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     4501 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     4561 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     4621 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     4681 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     4741 tattgtgctg tctcatcatt ttggcaaaga attgatttga taccgcgggc ccgggatctc
     4801 gaaccggtgc caccatggtg gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     4861 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     4921 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     4981 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     5041 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     5101 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     5161 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     5221 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     5281 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     5341 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     5401 agcccggtgc caaacagaaa attgtggcac cagtgaaaca gactttgaat tttgaccttc
     5461 tcaagttggc gggagacgtc gagtccaacc ctgggcccgg cccggtcgcc accatggtga
     5521 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     5581 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     5641 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     5701 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     5761 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     5821 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     5881 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     5941 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     6001 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     6061 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     6121 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     6181 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taggcggccg
     6241 caagccttgt taagtgctcg cttcggcagc acatatacta tgttgaatga ggcttcagta
     6301 ctttacagaa tcgttgcctg cacatcttgg aaacacttgc tgggattact tcttcaggtt
     6361 aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgagc tcccgtgaat
     6421 tggaatccta gtgaagccac agatgtagga ttccaattca gcgggagcct gcctactgcc
     6481 tcggaattca aggggctact ttaggagcaa ttatcttgtt tactaaaact gaataccttg
     6541 ctatctcttt gatacatttt tacaaagctg aattaaaatg gtataaatta aatcactttt
     6601 ttcaattgga agactaatgc gtttaaacca catgtttaag ggttccggtt ccactaggta
     6661 caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     6721 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6781 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6841 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6901 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6961 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7021 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7081 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7141 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7201 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7261 gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat
     7321 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     7381 ggaggaggtg ggttttccag tcacacctca ggtaccaagc atggggtaaa gtactgttct
     7441 catcacatca tatcaaggtt atataccatc aatattgcca cagatgttac ttagcctttt
     7501 aatatttctc taatttagtg tatatgcaat gatagttctc tgatttctga gattgagttt
     7561 ctcatgtgta atgattattt agagtttctc tttcatctgt tcaaattttt gtctagtttt
     7621 attttttact gatttgtaag acttcttttt ataatctgca tattacaatt ctctttactg
     7681 gggtgttgca aatattttct gtcattctat ggcctgactt ttcttaatgg ttttttaatt
     7741 ttaaaaataa gtcttaatat tcatgcaatc taattaacaa tcttttcttt gtggttagga
     7801 ctttgagtca taagaaattt ttctctacac tgaagtcatg atggcatgct tctatattat
     7861 tttctaaaag atttaaagtt ttgccttctc catttagact tataattcac tggaattttt
     7921 ttgtgtgtat ggtatgacat atgggttccc ttttattttt tacatataaa tatatttccc
     7981 tgtttttcta aaaaagaaaa agatcatcat tttcccattg taaaatgcca tatttttttc
     8041 ataggtcact tacatatatc aatgggtctg tttctgagct ctactctatt ttatcagcct
     8101 cactgtctat ccccacacat ctcatgcttt gctctaaatc ttgatattta gtggaacatt
     8161 ctttcccatt ttgttctaca agaatatttt tgttattgtc ttttgggctt ctatatacat
     8221 tttagaatga ggttggcaag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     8281 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc cagctgcttt
     8341 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8401 tagggaaccc actgcttaag cctcataaag ctcgactgtg ccttctagtt gccagccatc
     8461 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     8521 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     8581 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     8641 ggatgcggtc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8701 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8761 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8821 tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8881 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8941 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9001 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9061 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9121 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9181 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9241 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9301 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     9361 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9421 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9481 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9541 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9601 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9661 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9721 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9781 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9841 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9901 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9961 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    10021 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    10081 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    10141 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    10201 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    10261 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    10321 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    10381 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    10441 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10501 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10561 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10621 gttccgcgca catttccccg aaaagtgcca cctgac
//
