LOCUS       sequence-93590-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 513, Emission:
            527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: YPet-pBAD
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             55..77
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             97..114
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             118..150
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             163..183
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(496..515)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttatcgcaac tctctactgt ttctccatac ccgttttttt gggctagaaa taattttgtt
       61 taactttaag aaggagatat acatatgcgg ggttctcatc atcatcatca tcatggtatg
      121 gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgagaacct gtacttccag
      181 ggctcgagca tggtgagcaa aggcgaagag ctgttcaccg gcgtggtgcc catcctggtg
      241 gagctggacg gcgacgtgaa cggccacaag ttcagcgtga gcggcgaggg cgagggcgac
      301 gccacctacg gcaagctgac cctgaagctg ctgtgcacca ccggcaagct gcccgtgccc
      361 tggcccaccc tggtgaccac cctgggctac ggcgtgcagt gcttcgcccg gtaccccgac
      421 cacatgaagc agcacgactt cttcaagagc gccatgcccg agggctacgt gcaggagcgg
      481 accatcttct tcaaggacga cggcaactac aagacccggg ccgaggtgaa gttcgagggc
      541 gacaccctgg tgaaccggat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      601 ctgggccaca agctggagta caactacaac agccacaacg tgtacatcac cgccgacaag
      661 cagaagaacg gcatcaaggc caacttcaag atccggcaca acatcgagga cggcggcgtg
      721 cagctggccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      781 gacaaccact acctgagcta ccagagcgcc
//
