LOCUS       sequence-93594-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene 
            transfer, hypercholesterolemia and atherosclerosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Bjorklund MM, Hollensen AK, Hagensen MK, Dagnaes-Hansen F, 
            Christoffersen C, Mikkelsen JG, Bentzon JF
  TITLE     Induction of atherosclerosis in mice and hamsters without germline 
            genetic engineering.
  JOURNAL   Circ Res. 2014 May 23;114(11):1684-9. doi: 
            10.1161/CIRCRESAHA.114.302937. Epub 2014 Mar 27.
  PUBMED    24677271
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1161/CIRCRESAHA.114.302937"; journalName: "Circ Res"; date: 
            "2014-05-23- 23"; volume: "114"; issue: "11"; pages: "1684-9"
COMMENT     Sequence Label: pAAV/D374Y-hPCSK9
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      197..206
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             788..799
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cagcagcctc ccccgttgcc cctctggatc cactgcttaa atacggacga ggacagggcc
       61 ctgtctcctc agcttcaggc accaccactg acctgggaca gtgaatgatc cccctgatct
      121 gcggcctcga cggtatcgat aagcttgata tcgaattcta gtcgtcgacc actttcacaa
      181 tctgcggcac cggtgcgcca ccatgggcac cgtcagctcc aggcggtcct ggtggccgct
      241 gccactgctg ctgctgctgc tgctgctcct gggtcccgcg ggcgcccgtg cgcaggagga
      301 cgaggacggc gactacgagg agctggtgct agccttgcgt tccgaggagg acggcctggc
      361 cgaagcaccc gagcacggaa ccacagccac cttccaccgc tgcgccaagg atccgtggag
      421 gttgcctggc acctacgtgg tggtgctgaa ggaggagacc cacctctcgc agtcagagcg
      481 cactgcccgc cgcctgcagg cccaggctgc ccgccgggga tacctcacca agatcctgca
      541 tgtcttccat ggccttcttc ctggcttcct ggtgaagatg agtggcgacc tgctggagct
      601 ggccttgaag ttgccccatg tcgactacat cgaggaggac tcctctgtct ttgcccagag
      661 catcccgtgg aacctggagc ggattacccc tccacggtac cgggcggatg aataccagcc
      721 ccccgacgga ggcagcctgg tggaggtgta tctcctagac accagcatac agagtgacca
      781 ccgggaaatc gagggcaggg tcatggt
//
