LOCUS       sequence-93595-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: SP6-VEE-OKS-iM
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(267..320)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gtctctctcc gaggtagggg cgccaggttg ctaccgccgc aagccgcacc ggctccgccg
       61 ctctccaggt ctgtggccac ggtcgccgcc gccaggtcat aggggcggcc gggaagcact
      121 gggggaagtc gcttcatgtg ggagagctcc tcccgccagc ggttattcgg ggcacctgct
      181 tgacgcagtg tcttctccct tcccgccggg ccagacgcga acgtggagaa agatgggagc
      241 agcgcgtcgc tgacagccat caattgtggg ccaggattct cctcgacgtc accgcatgtt
      301 agcagacttc ctctgccctc actagagttt gaatgcatgg gagagcccag agtggtgacg
      361 gagacagggg gaaaggcttc cccctcaggg aaagggaccg aggagtacag tgcagtgaag
      421 tgagggctcc catagcctgg ggtaccaaaa tggggccctg gggccagagg aaaggacact
      481 ggtccccctg agaaaggaga cccagcagcc tcaaaatcct ctcgttgtgc atagtcgctg
      541 cttgatcgct tgcccttctg gcgccggtta cagaaccaca ctcggaccac atccttctcg
      601 agcccaagct gctgggcgat gtggctgatc tgctgcagtg tgggtttcgg gcactgcagg
      661 aacaaattct ccaggttgcc tctcactcgg ttctcgatac tggttcgctt tctctttcgg
      721 gcctgcacga gggtttctgc tttgcatatc tcctgaagat tttcattgtt gtcagcttcc
      781 tccacccact tctgcagcaa gggccgcagc ttacacatgt tcttgaagct aagctgcaga
      841 gcctc
//
