LOCUS       sequence-93598-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: SP6-VEE-OKS-iM
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      641..650
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcattgttgt cagcttcctc cacccacttc tgcagcaagg gccgcagctt acacatgttc
       61 ttgaagctaa gctgcagagc ctcaaagcgg cagatggtcg tttggctgaa taccttccca
      121 aatagaaccc ccagggtgag ccccacatcg gcctgtgtat atcccagggt gatcctcttc
      181 tgcttcagga gcttggcaaa ttgctcgagt tctttctgca gagctttgat gtcctgggac
      241 tcctccgggt tttgctccag cttctccttc tccagcttca cggcaccagg ggtgacggtg
      301 cagggctccg gggaggcccc atcggagttg ctctccaccc cgactcctgc ttcgccctca
      361 ggctgagagg tctccaagcc gccttggggc actagcccca ctccaacctg gggcccacag
      421 tacgccatcc ccccacagaa ctcatacggc ggggggcatg ggggaatccc ccacacctca
      481 gagcctggcc caacccccgg cccgattcct ggccctccag gagggccttg gaagcttagc
      541 caggtccgag gatcaaccca gcccggctcc ggcccccctg gcccatcacc tccaccacct
      601 ggagggggcg agaaggcgaa atccgaagcc aggtgtcccg ccatggtggc gaattcacgc
      661 gcccatatgc tagacttggc ggactagact atgtcgtagt ccattcaggt tagccgtaga
      721 gagttatagg ggcccctctc aggtagctga atgatttaac actgctagct agagtagtca
      781 tggccataac tatgatggaa gttcctacgg
//
