LOCUS       sequence-9360-ex       10849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO
  TITLE     A system for Cre-regulated RNA interference in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.
  PUBMED    18779577
REFERENCE   2  (bases 1 to 10849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Sep 16. 105(37):13895-900."
COMMENT     Sequence Label: pLB2 Ubi-FLIP FF
FEATURES             Location/Qualifiers
     source          1..10849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    489..510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(525..530,531..548,549..555)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    563..579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     568..590
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     587..603
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     587..603
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             869..1049
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1096..1221
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1720..1953
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2138..2182
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2480..2597
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3054..4264
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4101..4120
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(4305..4338)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(4305..4338)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             4355..4951
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4355..4374)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4851..4871
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4982..5047
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             5048..5536
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     protein_bind    complement(5547..5580)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     ncRNA           complement(5610..5736)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(5764..5778)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(5805..5926)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(join(5976..6689,6690..6692,6693..6695))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5979..6689,6690..6692,6693..6695))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6021..6042)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6370..6389
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6629..6650
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6692..6701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6709..6742
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    6709..6742
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    6789..6822
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    6878..7466
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6931..6951)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7349..7360)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7469..7485)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7470..7486)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    8629..8836
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     LTR             8855..9035
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9097..9685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9177..9196)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9856..10647,10648..10716))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10479..10498
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10717..10821)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagtcat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      301 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
      361 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
      421 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      481 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      541 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
      601 gaccatgatt acgccaagcg cgcaattaac cctagcttaa tgtagtctta tgcaatactc
      661 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
      721 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
      781 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
      841 tttaagtgcc tagctcgata caataaacgg gtctctctgg ttagaccaga tctgagcctg
      901 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      961 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1021 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     1081 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     1141 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     1201 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     1261 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     1321 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1381 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1441 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1501 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1561 gccggccgcg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1621 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1681 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1741 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1801 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1861 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1921 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1981 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     2041 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     2101 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     2161 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     2221 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     2281 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     2341 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2401 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa
     2461 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     2521 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2581 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggttagtac
     2641 cgggcccggt gctttgctct gagccagccc accagtttgg aatgactcct ttttatgact
     2701 tgaattttca agtataaagt ctagtgctaa atttaatttg aacaactgta tagtttttgc
     2761 tggttggggg aaggaaaaaa aatggtggca gtgttttttt cagaattaga agtgaaatga
     2821 aaacttgttg tgtgtgagga tttctaatga catgtggtgg ttgcatactg agtgaagccg
     2881 gtgagcattc tgccatgtca ccccctcgtg ctcagtaatg tactttacag aaatcctaaa
     2941 ctcaaaagat tgatataaac catgcttctt gtgtatatcc ggtctcttct ctgggtagtc
     3001 tcactcagcc tgcatttctg ccagggcccg ctctagatct agacggttga tctggcctcc
     3061 gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc
     3121 agacgaaggg cgcaggagcg ttcctgatcc ttccgcccgg acgctcagga cagcggcccg
     3181 ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga
     3241 cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta
     3301 gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata
     3361 taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt
     3421 cttgtttgtg gatcgctgtg atcgtcactt ggtgagttgc gggctgctgg gctggccggg
     3481 gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc
     3541 tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcacaaaa
     3601 tggcggctgt tcccgagtct tgaatggaag acgcttgtaa ggcgggctgt gaggtcgttg
     3661 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     3721 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     3781 gtttgtcact gactggagaa ctcgggtttg tcgtctggtt gcgggggcgg cagttatgcg
     3841 gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg cctcgtcgtg tcgtgacgtc
     3901 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     3961 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     4021 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     4081 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     4141 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     4201 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     4261 agacgaagta cgcgctagcc gttaataagc ctcgatgcgg atccataact tcgtatagga
     4321 taccttatac gaagttatct caggtaccgc caccatgacc gagtacaagc ccacggtgcg
     4381 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     4441 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     4501 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     4561 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4621 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     4681 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     4741 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     4801 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     4861 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     4921 cacctggtgc atgacccgca agcccggtgc cctgtacaag aaacagaaaa ttgtggcacc
     4981 agtgaaacag actttgaatt ttgaccttct caagttggcg ggagacgtcg agtccaaccc
     5041 tgggcccatg aacccagcca tcagcgtcgc tctcctgctc tcagtcttgc aggtgtcccg
     5101 agggcagaag gtgaccagcc tgacagcctg cctggtgaac caaaaccttc gcctggactg
     5161 ccgccatgag aataacacca aggataactc catccagcat gagttcagcc tgacccgaga
     5221 gaagaggaag cacgtgctct caggcaccct cgggataccc gagcacacgt accgctcccg
     5281 cgtcaccctc tccaaccagc cctatatcaa ggtccttacc ctagccaact tcaccaccaa
     5341 ggatgagggc gactactttt gtgagcttcg agtctcgggc gcgaatccca tgagctccaa
     5401 taaaagtatc agtgtgtata gagacaaact ggtcaagtgt ggcggcataa gcctgctggt
     5461 tcagaacaca tcctggatgc tgctgctgct gctttccctc tccctcctcc aagccctgga
     5521 cttcatttct ctgtgatcta gaagccataa cttcgtatag tacacattat acgaagttat
     5581 gtttaaacgc attagtcttc caattgaaaa aagtgattta atttatacca ttttaattca
     5641 gctttgtaaa aatgtatcaa agagatagca aggtattcag ttttagtaaa caagataatt
     5701 gctcctaaag tagccccttg aattccgagg cagtaggcag gctcccgctg aattggaatc
     5761 ctacatctgt ggcttcacta ggattccaat tcacgggagc tcgctcactg tcaacagcaa
     5821 tataccttct cgagccttct gttgggttaa cctgaagaag taatcccagc aagtgtttcc
     5881 aagatgtgca ggcaacgatt ctgtaaagta ctgaagcctc attcaaacat agtatatgtg
     5941 ctgccgaagc gagcacttaa caaggcttgc ggccgctact tgtacagctc gtccatgccg
     6001 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     6061 gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg
     6121 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg
     6181 atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata
     6241 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
     6301 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
     6361 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
     6421 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
     6481 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
     6541 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
     6601 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
     6661 accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtat aacttcgtat
     6721 aaggtatcct atacgaagtt atccattcag gctgtgctag catcaatggc atggcacaaa
     6781 gcttagccat aacttcgtat aatgtgtact atacgaagtt atccctgttt aagggttccg
     6841 gttccactag gtacaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     6901 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6961 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7021 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7081 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7141 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7201 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7261 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     7321 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7381 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     7441 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa
     7501 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     7561 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacca agcatggggt
     7621 aaagtactgt tctcatcaca tcatatcaag gttatatacc atcaatattg ccacagatgt
     7681 tacttagcct tttaatattt ctctaattta gtgtatatgc aatgatagtt ctctgatttc
     7741 tgagattgag tttctcatgt gtaatgatta tttagagttt ctctttcatc tgttcaaatt
     7801 tttgtctagt tttatttttt actgatttgt aagacttctt tttataatct gcatattaca
     7861 attctcttta ctggggtgtt gcaaatattt tctgtcattc tatggcctga cttttcttaa
     7921 tggtttttta attttaaaaa taagtcttaa tattcatgca atctaattaa caatcttttc
     7981 tttgtggtta ggactttgag tcataagaaa tttttctcta cactgaagtc atgatggcat
     8041 gcttctatat tattttctaa aagatttaaa gttttgcctt ctccatttag acttataatt
     8101 cactggaatt tttttgtgtg tatggtatga catatgggtt cccttttatt ttttacatat
     8161 aaatatattt ccctgttttt ctaaaaaaga aaaagatcat cattttccca ttgtaaaatg
     8221 ccatattttt ttcataggtc acttacatat atcaatgggt ctgtttctga gctctactct
     8281 attttatcag cctcactgtc tatccccaca catctcatgc tttgctctaa atcttgatat
     8341 ttagtggaac attctttccc attttgttct acaagaatat ttttgttatt gtcttttggg
     8401 cttctatata cattttagaa tgaggttggc aaggtacctt taagaccaat gacttacaag
     8461 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     8521 tcccagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     8581 ctctctggct aactagggaa cccactgctt aagcctcata aagctcgact gtgccttcta
     8641 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     8701 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     8761 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     8821 gcaggcatgc tggggatgcg gtccggactg tactgggtct ctctggttag accagatctg
     8881 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8941 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9001 cagacccttt tagtcagtgt ggaaaatctc tagcagcatg tgagcaaaag gccagcaaaa
     9061 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9121 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9181 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9241 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9301 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9361 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9421 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9481 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9541 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9601 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9661 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9721 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9781 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9841 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9901 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9961 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10021 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10081 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10141 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10201 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10261 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10321 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10381 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10441 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10501 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10561 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10621 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10681 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10741 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10801 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
