LOCUS       sequence-93602-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene
            transfer, hypercholesterolemia and atherosclerosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Bjorklund MM, Hollensen AK, Hagensen MK, Dagnaes-Hansen F, 
            Christoffersen C, Mikkelsen JG, Bentzon JF
  TITLE     Induction of atherosclerosis in mice and hamsters without germline 
            genetic engineering.
  JOURNAL   Circ Res. 2014 May 23;114(11):1684-9. doi: 
            10.1161/CIRCRESAHA.114.302937. Epub 2014 Mar 27.
  PUBMED    24677271
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1161/CIRCRESAHA.114.302937"; journalName: "Circ Res"; date: 
            "2014-05-23- 23"; volume: "114"; issue: "11"; pages: "1684-9"
COMMENT     Sequence Label: pAAV/D377Y-mPCSK9
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             792..803
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cagcagcctc ccccgttgcc cctctggatc cactgcttaa atacggacga ggacagggcc
       61 ctgtctcctc agcttcaggc accaccactg acctgggaca gtgaatgatc cccctgatct
      121 gcggcctcga cggtatcgat aagcttgata tcgaattcta gtcgtcgacc actttcacaa
      181 tctgcggcac cggtaccatg ggcacccact gctctgcgtg gctgcggtgg ccgctgttgc
      241 cgctgttgcc gccgctgctg ctgctgttgc tgctactgtg ccccaccggc gctggtgccc
      301 aggacgagga tggagattat gaagagctga tgctcgccct cccgtcccag gaggatggcc
      361 tggctgatga ggccgcacat gtggccaccg ccaccttccg ccgttgctcc aaggaggcct
      421 ggaggctgcc aggaacctac attgtggtgc tgatggagga gacccagagg ctacagattg
      481 aacaaactgc ccaccgcctg cagacccggg ctgcccgccg gggctatgtc atcaaggttc
      541 tacatatctt ttatgacctc ttccctggct tcttggtgaa gatgagcagt gacctgttgg
      601 gcctggccct gaagttgccc catgtggagt acattgagga agactccttt gtcttcgccc
      661 agagcatccc atggaacctg gagcgaatta tcccagcatg gcaccagaca gaggaagacc
      721 gctcccctga tggaagcagc caggtggagg tgtatctctt agataccagc atccagggtg
      781 cccatcggga gattgagggc agggtcacca tcaccgactt caaca
//
