LOCUS       sequence-93608-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: SP6-VEE-OKS-iG
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(268..321)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggtctctctc cgaggtaggg gcgccaggtt gctaccgccg caagccgcac cggctccgcc
       61 gctctccagg tctgtggcca cggtcgccgc cgccaggtca taggggcggc cgggaagcac
      121 tgggggaagt cgcttcatgt gggagagctc ctcccgccag cggttattcg gggcacctgc
      181 ttgacgcagt gtcttctccc ttcccgccgg gccagacgcg aacgtggaga aagatgggag
      241 cagcgcgtcg ctgacagcca tcaattgtgg gccaggattc tcctcgacgt caccgcatgt
      301 tagcagactt cctctgccct cactagagtt tgaatgcatg ggagagccca gagtggtgac
      361 ggagacaggg ggaaaggctt ccccctcagg gaaagggacc gaggagtaca gtgcagtgaa
      421 gtgagggctc ccatagcctg gggtaccaaa atggggccct ggggccagag gaaaggacac
      481 tggtccccct gagaaaggag acccagcagc ctcaaaatcc tctcgttgtg catagtcgct
      541 gcttgatcgc ttgcccttct ggcgccggtt acagaaccac actcggacca catccttctc
      601 gagcccaagc tgctgggcga tgtggctgat ctgctgcagt gtgggtttcg ggcactgcag
      661 gaacaaattc tccaggttgc ctctcactcg gttctcgata ctggttcgct ttctctttcg
      721 ggcctgcacg agggtttctg ctttgcatat ctcctgaaga ttttcattgt tgtcagcttc
      781 ctccacccac ttctgca
//
