LOCUS       sequence-9362-ex       10079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   Stadtfeld M, Maherali N, Breault DT, Hochedlinger K
  TITLE     Defining molecular cornerstones during fibroblast to iPS cell 
            reprogramming in mouse.
  JOURNAL   Cell Stem Cell. 2008 Mar 6. 2(3):230-40.
  PUBMED    18371448
REFERENCE   2  (bases 1 to 10079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2008 Mar 6. 2(3):230-40."
COMMENT     Sequence Label: pLV-tetO-Klf4
FEATURES             Location/Qualifiers
     source          1..10079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2610..2628,2629..2651,2652..2670,2671..2693,2694..2712,
                     2713..2735,2736..2754,2755..2777,2778..2796,2797..2819,
                     2820..2838,2839..2861,2862..2880)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2913..2950
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2947..2971
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3056..3079
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4771..5359
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4824..4844)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5242..5253)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5362..5378)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5363..5379)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5884..6064
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6090..6107)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6096..6320
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6366..6794
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6453..6472)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6663..6684
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6803..6823)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6808..7137
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6988..7123
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7050..7069
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7185..7232
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7251..7625
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7755..7876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7792..7811)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7846..7865
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7925..7941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7925..7941)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7938..7960)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7949..7965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7973..7979,7980..7997,7998..8003))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8018..8039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8156..8173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8327..8915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8407..8426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9086..9877,9878..9946))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9709..9728
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9947..10051)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2881 aaagtgaaag tcgagctcgg tacccgggtc gagtaggcgt gtacggtggg aggcctatat
     2941 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     3001 cctccataga agacaccggg accgatccag cctccgcggc cccgaattca ccatggacta
     3061 caaagacgat gacgacaagc ttatgaggca gccacctggc gagtctgaca tggctgtcag
     3121 cgacgctctg ctcccgtcct tctccacgtt cgcgtccggc ccggcgggaa gggagaagac
     3181 actgcgtcca gcaggtgccc cgactaaccg ttggcgtgag gaactctctc acatgaagcg
     3241 acttccccca cttcccggcc gcccctacga cctggcggcg acggtggcca cagacctgga
     3301 gagtggcgga gctggtgcag cttgcagcag taacaacccg gccctcctag cccggaggga
     3361 gaccgaggag ttcaacgacc tcctggacct agactttatc ctttccaact cgctaaccca
     3421 ccaggaatcg gtggccgcca ccgtgaccac ctcggcgtca gcttcatcct cgtcttcccc
     3481 agcgagcagc ggccctgcca gcgcgccctc cacctgcagc ttcagctatc cgatccgggc
     3541 cgggggtgac ccgggcgtgg ctgccagcaa cacaggtgga gggctcctct acagccgaga
     3601 atctgcgcca cctcccacgg cccccttcaa cctggcggac atcaatgacg tgagcccctc
     3661 gggcggcttc gtggctgagc tcctgcggcc ggagttggac ccagtataca ttccgccaca
     3721 gcagcctcag ccgccaggtg gcgggctgat gggcaagttt gtgctgaagg cgtctctgac
     3781 cacccctggc agcgagtaca gcagcccttc ggtcatcagt gttagcaaag gaagcccaga
     3841 cggcagccac cccgtggtag tggcgcccta cagcggtggc ccgccgcgca tgtgccccaa
     3901 gattaagcaa gaggcggtcc cgtcctgcac ggtcagccgg tccctagagg cccatttgag
     3961 cgctggaccc cagctcagca acggccaccg gcccaacaca cacgacttcc ccctggggcg
     4021 gcagctcccc accaggacta cccctacact gagtcccgag gaactgctga acagcaggga
     4081 ctgtcaccct ggcctgcctc ttcccccagg attccatccc catccggggc ccaactaccc
     4141 tcctttcctg ccagaccaga tgcagtcaca agtcccctct ctccattatc aagagctcat
     4201 gccaccgggt tcctgcctgc cagaggagcc caagccaaag aggggaagaa ggtcgtggcc
     4261 ccggaaaaga acagccaccc acacttgtga ctatgcaggc tgtggcaaaa cctataccaa
     4321 gagttctcat ctcaaggcac acctgcgaac tcacacaggc gagaaacctt accactgtga
     4381 ctgggacggc tgtgggtgga aattcgcccg ctcggatgaa ctgaccaggc actaccgcaa
     4441 acacacaggg caccggccct ttcagtgcca gaagtgcgac agggcctttt ccaggtcgga
     4501 ccaccttgcc ttacacatga agaggcactt ttaaatccca cgtagtggat gtgacccaca
     4561 ctgccaggag agagagttca gtattttttt ttctaacctt tcacactgtc ttcccacgag
     4621 gggaggagcc cagctggcaa gcgctacaat catggtcaag ttcccagcaa gtcagcttgt
     4681 gaatggataa tcaggagaaa ggaagagtcc aagagacaaa acagaaatac taaaaacaaa
     4741 caaacggaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     4801 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4861 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4921 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4981 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5041 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5101 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5161 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     5221 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5281 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5341 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
     5401 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     5461 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     5521 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     5581 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     5641 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     5701 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     5761 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     5821 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     5881 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5941 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6001 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6061 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     6121 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6181 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     6241 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     6301 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     6361 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6421 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6481 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6541 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6601 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6661 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6721 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6781 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     6841 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     6901 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6961 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7021 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     7081 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     7141 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     7201 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     7261 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     7321 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     7381 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     7441 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     7501 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     7561 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     7621 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     7681 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     7741 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7801 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7861 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     7921 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7981 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8041 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8101 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8161 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8221 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8281 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8341 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8401 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8461 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8521 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8581 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8641 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8701 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8761 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8821 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8881 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8941 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9001 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9061 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9121 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9181 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9241 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9301 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9361 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9421 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9481 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9541 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9601 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9661 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9721 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9781 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9841 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9901 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9961 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10021 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
